An . imagej. . plugin. with cell tracking capabilities. Acquiring Manual Tracking. The first step to acquiring the manual tracking . plugin. is searching “. imagej. cell tracking” and clicking the first link.. ID: 337886
DownloadNote - The PPT/PDF document "Manual Tracking" is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Presentations text content in Manual Tracking
Slide1
Manual Tracking
An
imagej
plugin
with cell tracking capabilities
Slide2
Acquiring Manual Tracking
The first step to acquiring the manual tracking plugin is searching “imagej cell tracking” and clicking the first link.Once on the ibidi page select “Manual Tracking” from the left menu containing Product CategoriesFrom this page click the link shown below
Slide3
Acquiring Manual Tracking(cont.)
The next step is to download Manual Tracking from the purple link shown and move this file to the plugins folder of imagej
Slide4
Using Manual Tracking
Once in imagej, open the zvi file you wish to track.Click the Plugins button and select Manual Tracking in the drop down menuA new window should open. This window allows you to set the parameters for the track you are about to make.The time interval and x/y calibration values can be selected here. A z value can also be calibrated if 3D tracking is being done.
Slide5
Using Manual Tracking
The Add track button in the top left should then be clicked.Clicking on the nucleus will now produce xy points that pop up in a new window. If you continue to track the cell then the Manual Tracking program will produce distance and velocity values for the two points.
Slide6
Further Information
The two following links offer guidelines to doing other operations with the manual tracking
Download this presentation
DownloadNote - The PPT/PDF document "Manual Tracking" is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Presentations text content in Manual Tracking
Manual Tracking
An
imagej
plugin
with cell tracking capabilities
Slide2Acquiring Manual Tracking
The first step to acquiring the manual tracking plugin is searching “imagej cell tracking” and clicking the first link.Once on the ibidi page select “Manual Tracking” from the left menu containing Product CategoriesFrom this page click the link shown below
Slide3Acquiring Manual Tracking(cont.)
The next step is to download Manual Tracking from the purple link shown and move this file to the plugins folder of imagej
Slide4Using Manual Tracking
Once in imagej, open the zvi file you wish to track.Click the Plugins button and select Manual Tracking in the drop down menuA new window should open. This window allows you to set the parameters for the track you are about to make.The time interval and x/y calibration values can be selected here. A z value can also be calibrated if 3D tracking is being done.
Slide5Using Manual Tracking
The Add track button in the top left should then be clicked.Clicking on the nucleus will now produce xy points that pop up in a new window. If you continue to track the cell then the Manual Tracking program will produce distance and velocity values for the two points.
Slide6Further Information
The two following links offer guidelines to doing other operations with the manual tracking
plugin
http://
rsb.info.nih.gov/ij/plugins/track/track.html
http://
rsb.info.nih.gov/ij/plugins/track/Manual%20Tracking%20plugin.pdf
Slide7Slide8Slide9Slide10Slide11