PDF-Proc.Natl.Acad.Sci.USAVol.95,pp.14717

Author : tatyana-admore | Published Date : 2016-08-25

Wnt1isamemberofanexpandingfamilyofcysteinerichglycosylatedsignalingproteinsthatmediatediversedevelopmentalprocessessuchasthecontrolofcellproliferationadhesioncellpolarityandtheestablishmentofce

Presentation Embed Code

Download Presentation

Download Presentation The PPT/PDF document "Proc.Natl.Acad.Sci.USAVol.95,pp.14717" is the property of its rightful owner. Permission is granted to download and print the materials on this website for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.

Proc.Natl.Acad.Sci.USAVol.95,pp.14717: Transcript

Wnt1isamemberofanexpandingfamilyofcysteinerichglycosylatedsignalingproteinsthatmediatediversedevelopmentalprocessessuchasthecontrolofcellproliferationadhesioncellpolarityandtheestablishmentofce. Proc.Natl.Acad.Sci.USA90(1993)10391days.Whenthecellswereforamicroinjectionexperiment,coverslipswerefirstmarkedwithadiamond-tippentofacilitatethelocalizationandvisualizationofmicroinjectedcells.After8- Proc.Natl.Acad.Sci.USA90(1993)424Age(months)200.0120z0.010laaCD-_-0.008oo100CQ0.006coCD~0o0.004x0NuclearDNATI0.002F0.04242a100000Q0la)co0x0Age(months)FIG.1.Steady-stateoxidativeDNAdamageincreaseswitha Proc.Natl.Acad.Sci.USA90(1993)11479knownconcentration.DNAwasstoredin1mMTris,pH7.8/0.1mMEDTA.Forinjections,DNAwasdilutedinsaline(0.9%NaCl).VaccineTrials.Vaccinetrialsinmicewereaccomplishedbytheadminist Proc.Natl.Acad.Sci.USA93(1996)9293exonjunctionsinthespy-1andspy-2mutants,genomicDNAwassubjectedtoPCRandtheproductsweresequenceddirectly.RESULTSANDDISCUSSIONIsolationofspy-4.Thespy-4mutantwasisolatedby Proc.Natl.Acad.Sci.USA92(1995)7141FRETefficiencyRNAbulgeA7AgFIG.2.KinkingofDNAandRNAduplexesbybasebulgesisrevealedbyFRET.Thedistancebetweendonor(D)andacceptor(A)fluorophoresattachedtothe5'terminiofthe 868CellBiology:OsbornandWeberFIGS.1-7.(Legendappearsatbottomofthefollowingpage.)Proc.Nat.Acad.Sci.USA73(1976) Proc.Nat.Acad.Sci.USA73(1976)869FIG.8.Recoveryofcytoplasmicmicrotubulesin3T3cellsafterColc Proc.Natl.Acad.Sci.USA75(1978)RESULTSWhennormalhumanglobinDNAwasdigestedwithEcoRIandprobedwithamixtureofa-and(3-globincDNAs,fivemajorfragmentswereseen(Fig.1).Thelargestfragment,about21kilobases(kb)inl Proc.Nat.Acad.Sci.USA70(1978)624~~~~~~~~~~~~24*0aS5!!402040608000020-tO60eo0as--40506~~~~~~~~~~~~~0GROWTHINTHEL00H000)CRW'0NTE0~Z04-20FIG.2.(A)Kineticsofinductionofthechloroplastisoleucyl-tRNAuponexpo Proc.Natl.Acad.Sci.USA93(1996)11529BothLandL*canbeprotonatedonLys-156,butthepKaforprotonationisconsiderablylowerforL*thanforL;pKL*iswhilepKLis10.OneimportanttenetofthismodelisthattheIndsmutationsincre Proc.Natl.Acad.Sci.USA90(1993)2031dues,0.98forLexA-C52containingasinglecysteine,and0againforLexA-C52aftermodificationwith4-azidophenacylbromide,suggestingthatessentiallyallthecysteinesidechainshaverea I,fromFlavobacteriumokeanokoites,isamemberoftheunusual,typeIIsclassofrestrictionendonucleasesthatrec-ognizeaspecificDNAsequenceandcleavenonspecificallyashortdistanceawayfromthatsequence(1).Ibindstheco Proc.Natl.Acad.Sci.USA93(1996)2583multiplexamplificationasdescribedabove.Theonlydiffer-encebetweenthesetwoproceduresisthatsomePprimersusedinMGSmaycontainsingle-basemismatchestothetemplatestoconvertthe Proc.Natl.Acad.Sci.USA92(1995)11999BacterialcellEukaryoticcellCalmodulinActive*-*tJ~'X-cyclaseATPcAMPFIG.1.Strategytoanalyzeinternalizationofbacterialproteinsinsidethecytosolofeukaryoticcells.sec,Gene Proc.Natl.Acad.Sci.USA88(1991)downstreamofthelacpromoterasfollows.PlasmidpHS202(5),whichcontainsnucleotides1-1010(thePvuII-KpnIchromosomefragmentthatencompassesruvA)intheSmaI-KpnIsiteofpUC19,wasdigest

Download Document

Here is the link to download the presentation.
"Proc.Natl.Acad.Sci.USAVol.95,pp.14717"The content belongs to its owner. You may download and print it for personal use, without modification, and keep all copyright notices. By downloading, you agree to these terms.