Professor Diane Hilker Exp 16 Identification of Unknown Microorganisms Overview TODAY Interpret Temp Study Exp 7Table1 Determine Optimum Temp Any contaminants in tubes ObserveDescribe Colony Morphology Plate ID: 1044086
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1. BIO 201 Lab 12Exp. 16: Week 2 UnknownsProfessor Diane Hilker
2. Exp. 16: Identification of Unknown MicroorganismsOverview
3. TODAY:Interpret Temp. Study (Exp. 7-Table1)Determine Optimum Temp.Any contaminants in tubes?Observe/Describe Colony Morphology PlateRepeat Gram Stain Does it agree with last week’s results?If need be, repeat Wet Mount & Simple StainSet up the necessary tests for YOU!Incubate at YOUR Optimum Temp.I. Exp. 16: Identification of Unknown Microorganisms
4. YeastOpt. Temp: 5, 25, 37oC Dry,white, crusty growthWet Mount: large, ovoid, irregular, budding, Brownian MovementSimple Stain: large, ovoid, irregular, buddingGram Stain: Same as above, cells stain either purple, pink or both Catalase: + (bubbles) No other testing necessaryI. Exp. 16: Identification of Unknown Microorganisms
5. Gram Pos. Cocci: Staph and StrepOpt. Temp: 25-37oC Small white creamy coloniesStaphylococcusGram Pos. cocci in clustersCatalase: +Halophile I. Exp. 16: Identification of Unknown Microorganisms
6. StreptococcusGram Pos. cocci in chainsCatalase: -Not a halophile Today: 1. Do Catalase test (Exp. 15) 2. Inoculate Salt Plate (Staph 110 or MSA) I. Exp. 16: Identification of Unknown Microorganisms
7. Gram Pos. Rod: Bacillus stearothermophilusOpt. Temp: 60oC Transparent beige coloniesSimple Stain: endosporesGram Pos. long rods. Cell wall breaks down quickly & looks pink if not observed in 24 hrs.I. Exp. 16: Identification of Unknown Microorganisms
8. Gram Pos. Rod: Bacillus stearothermophilusToday:Inoculate Starch Plate (Exp. 15): dab the sample in the center of the plateIncubate at 60oC in sealed plastic bagI. Exp. 16: Identification of Unknown Microorganisms
9. Gram Negative Rods: short rodsOpt. Temp: 25 -37oC Beige large colonies, may be motileE. coliPseudomonas-may have a little growth at 5oC, yellow-green pigmentation of mediaSerratia- pink at 25oCEnterobacterCitrobacterProteusI. Exp. 16: Identification of Unknown Microorganisms
10. Gram Negative RodsToday-Inoculate ALL of the following tubes (Exp. 15):PRLBPRDBTryptone BrothMRVP BrothUrea BrothSimmons Citrate SlantTSI Slant-Stab the bottom of the tube with inoculating needleI. Exp. 16: Identification of Unknown Microorganisms