PPT-Chapter 17: Introduction of DNA into eukaryotic cells – biological methods

Examples Viral transduction into mammalian cells Agrobacterium transformation of plant cells Sources httpenwikipediaorgwikiViralvector Daniel Eisenman Medical University of South Carolina. Kingdom Protista. A. Sometimes not considered a true kingdom because the organisms vary tremendously from one to another. The only universal characteristic among the group is that they are all eukaryotic.. 2.3.1, 2.3.2: Liver cell. 2.3.3: Electron micrograph of liver cell (rat). Glycogen. 2.3.4: Prokaryotic VS Eukaryotic cell. FEATURE. Prokaryotic cell. Eukaryotic cell. DNA. Naked DNA. DNA associated. with proteins. Eukaryotes, Prokaryotes and Measuring Cells. Lesson Objectives. To understand the difference between eukaryotic and prokaryotic cells.. To describe the features of a prokaryotic cell such as a bacterium.. Lily Chan and Tim Johnstone. Transfection:. . to transfer DNA into cells (either eukaryotic or prokaryotic) not through use of a viral vector. The approaches for eukaryotic and prokaryotic-cell . transfection are slightly . Lily Chan and Tim Johnstone. Transfection:. . to transfer DNA into cells (either eukaryotic or prokaryotic) not through use of a viral vector. The approaches for eukaryotic and prokaryotic-cell . transfection are slightly . Two Basic Types. Remember….cells are the basic unit of life for ALL living things.. There are two basic types of cells:. Prokaryotic cells . – found in bacteria. Eukaryotic cells . – found in . Multicellular. . Organisms. Overview. Review of key structural and genetic features of eukaryotic cells.. Remind yourself of these by your own reading. .. The origin of cells with eukaryotic organization, some 2.5 . AP Bio . Period 1&2. NUCLEUS AND RIBOSOMES. Found in eukaryotic plant and animal cells.. Located within the nucleus.. Is a network of condensed strands . - coil into rod-like structures called chromosomes during mitosis. Multicellular. . Organisms. Overview. Review of key structural and genetic features of eukaryotic cells.. Remind yourself of these by your own reading. .. The origin of cells with eukaryotic organization, some 2.5 . Unit. Context of Unit in Course . Unit is for a second year, intro cell biology or intro microbiology course, scalable to first year intro biology. This . unit comes in . the middle . of . the course. P & E UKARYOTIC C ELLS  As you have already learned, everything is made up of living cells.  And the cells themselves are made up of many different parts .  There are many different cell histones. (50%). Five major . histones. ; H2A, H2B, H3, H4 and H1 highly basic (. Arg. and Lys >25%). Roger Kornberg in 1974; . Chromatin. is made up of repeating units called . nucleosomes. , each containing 200 . A single guiding RNA (sgRNA; . a fusion of . tracrRNA. and crRNA. ) . binds CAS9 protein at one end, the other end guides CAS9 to target DNA sequence based on complementarity. . Gene for the CAS9 protein, encodes endonuclease, cuts both strands of the target sequence. An early developmental stage of a starfish. Kingdom . Animalia. Invertebrates. Some reproduce sexually, others asexually. Can regenerate from just one arm. B- Mammal Intestine. (Multicellular/Eukaryotic).