PDF-RNA immunoprecipitation (RIP)

protocol Guidance for running a n efficient and accurate experiment 2 RNA immunoprecipitatio n RIP protocol RIP is an antibody based technique used to map in vivo RNA protein interactions Th. melanogaster Xenopus zebra64257sh bovine Dg dog Pg pig Sc S cerevisiae Ce C elegans Hr Horse All all species expected Species enclosed in parentheses are predicted to react based on 100 homology For Research Use Only Not For Use In Diagnostic Proced Thoroughly resuspend the SureBeads in their solution and transfer 100 l 1 mg at 10 mgml of SureBeads to 15 ml tubes Magnetize beads and discard supernatant 2 Wash with 1000 l PBST PBS 01 Tween 20 a Resuspend the beads thoroughly b Magnetize beads a melanogaster Xenopus zebra64257sh bovine Dg dog Pg pig Sc S cerevisiae Ce C elegans Hr Horse All all species expected Species enclosed in parentheses are predicted to react based on 100 homology For Research Use Only Not For Use In Diagnostic Proced  2 age ......................................................................................................Materials Supplied by the User ................................................. The goal of chromatin immunoprecipitation (ChIP ) experiments is to map the binding sites of a molecule (usually a protein) across the genome in a cell type or tissue [ ] . ChIP assays start by cro  2 age ......................................................................................................Materials Supplied by the User ................................................. . 9620 Medical Center Drive, Suite 200, Rockville, MD 20850 www.origene.com techsupport@origene.com 1 - 301 - 340 - 3188 (P) 1 - 301 - 340 - 9254 (F) Page 1 rev 070512 Anti - DDK Magnetic Immu Immunoprecipitation Workflow and Troubleshooting Tips Bak/Bax . Deeksha Jain. Cancer . Apoptosis + Regulatory Factors. BCL-2 Regulators . MCL-1 . Amplifications in cancer . Myeloid . Cell Leukemia 1 (MCL-1) Pathway. Release of cytochrome c. Bax. Myeloid Cell Leukemia 1 .