PPT-Lab 2: Tissue Processing

Objective s What is tissue processing What are the major steps in tissue processing Identify types of fixative The advantage of each fixative The disadvantage of each fixative. Tissue Processing. Objective. s. What is . tissue processing. ?. What are the . major steps . in tissue processing?. Identify . types of fixative. .. The . advantage of each fixative. .. The . disadvantage of each fixative. Techniques. By. DR ANYANWU GE. Introduction. Histological technique deals with the preparation of tissue for microscopic examination. . The aim of good histological technique to preserve microscopic anatomy of tissue. . multi-wavelength . refractometer. Y.V. . Tarakanchikova. , L.E. . Dolotov. , A.P. Popov, . A.V. . Bykov. , V.V. . Tuchin. . Material and method. Determination of the refractive index is based on the Fresnel equations for the reflection of light using the FTIR. . Fixation. Tissues must be immersed in fixative immediately after removal from the body . . . . . 10. % Neutral Buffered Formalin is the routine fixative. . Tissue Processing. Tissue processing consists of 3 steps. HISTOLOGY :. . It is the branch of science which deals with the microscopic study of normal tissue . HISTOPATHOLOGY :. . It is the branch of science which deals with the microscopic study of tissue affected by disease. Form metabolic and structural connections between other tissues… nutrition and support. Forms a protective sheath around organs and helps insulate the body…cushion. Acts as a reserve for energy…storage. General Characteristics. Does have blood vessels- . variations in blood supply (ligaments and tendons don’t heal as fast because they are poorly vascularized). Cartilage is avascular –no blood supply- heals very slowly. “Application of principles and methods of engineering and life sciences toward fundamental understanding of structure-function relationships in normal and pathological mammalian tissues and the development of biological substitutes to restore, maintain or improve tissue conditions.”. Report in Summer PT's ready for height and weight @ 0530 inside BLDG 2730 (Rinehart Gym) with sponsor. . Ensure to have your orders, pre-execution check list, and WLC packing list (Including TA-50) on hand.. is the main component of the two parts of the . nervous system. ; the . brain. and . spinal cord. of the . central nervous system. (CNS), and the branching peripheral nerves of the . peripheral nervous system. It is the branch of science which deals with the gross & microscopic study of normal tissue. Histopathology:. It is the branch of science which deals with the gross & microscopic study of tissue affected by disease.. . . . . 10. % Neutral Buffered Formalin is the routine fixative. . Tissue Processing. Tissue processing consists of 3 steps. :. . 1-. Dehydration . . 2-. Clearing. . . Fixation. The sample should be placed in fixative (usually 10% neutral buffered formalin); the volume should be at least ten times that of the tissue.. The purpose of fixation is to preserve the skin specimen indefinitely in a life-like state. . Purpose Statement. At the end of the lecture student should be able to . describe. the . Composition of tissues. Histochemical. techniques. Learning Objectives . At the end of the lecture the student should be able to.