brPage 1br Bowdish Lab McMaster University Hamilton ON Canada brPage 2br ID: 88151
Download Pdf The PPT/PDF document "Bowdish Lab McMaster University Hamilton..." is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
ARVESTING OF EMURS AND IBIA FROM ICE Created/updated by:Mike DorringtonDate:04/07/11Bowdish Lab, McMaster University www.bowdish.ca ACKGROUNDThis protocol explains how to harvest the major leg bones from a mouse for the purpose of LEAN Tools should be sterilized with 70% EtOH and cleaned vigorously of all tissue and blood after use.Pins should be discarded into a sharps containerECIPES FOR EDIAR10 mediumRPMI1640500 ml etal Calf Serum55 ml1 M Hepes5 mlPenicillin/Streptomycin1 vial (5 ml), defrosted at 37C, containing 10 000 U Penicillin/ml; 10 mg/ml Streptomycin; 200 mM LGlutamineUsing 5 ml syringes, add the Hepes and Penicillin/Streptomycin to the RPMI through a 0.2m filter.Using a 50 ml syringe, add the Foetal Calf Serum through a syringe filter with 0.2m membrane.Store at 4C.