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Presentation Transcript
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Covaris truXTRAC – MALDI TOF MS Peptide Extraction for MALDI TOF MS<br>
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Cost Savings & Product Analysis<br>
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Comparing Sample Preparation Workflows for MALDI TOF MS Manual Workflow: 60-90 minutes Covaris truXTRAC Workflow: <5 minutes<br>
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Sample Preparation Workflow for BioMerieux Vitek MS Key Points to Address
Different sample inactivation methods (i.e., heat vs. mechanical disruption)
Different incubation times and reagent volumes used
The Covaris truXTRAC – MALDI TOF MS can be used as the extraction method for sample analysis on the BioMerieux MALDI TOF MS platform Manual Workflow: >40 minutes<br>
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Covaris truXTRAC – MALDI TOF MS Advantages<br>
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Adams, L., Dionne, K., Fisher, S., Parrish, N. A rapid standardized protein extraction method using adaptive focused acoustics for Identification of mycobacteria by MALDI TOF MS. DMID 2016; pii: S0732-8893(16)30161-4.doi:10.1016/j.diagmicrobio.2016.06.001. [Epub ahead of print] Publication Key Takeaways
Inactivation and peptide extraction from mycobacteria in ≤ 5 minutes with subsequent identification to the species level using MALDI TOF MS and a log confidence cut-off of ≥ 1.7
The AFA method does not require extensive library construction, but rather can be utilized for the most part with the existing commercial database
The time savings and decreased cost of AFA-based inactivation and peptide extraction for MALDI-ToF MS identification provides the means for a rapid, standardized protocol which can be utilized easily by clinical laboratories
Agreement for all species considered together was 100% regardless of the number of spots tested using a log confidence score of ≥ 1.7 and the existing Bruker database<br>
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Institutions Using truXTRAC – MALDI TOF MS in the Americas
Connecticut Department of Public Health
395 West Street
Rocky Hill, CT 06067
Contact Information Covaris, Inc. 14 Gill Street, Unit H Woburn, Massachusetts 01801
Telephone: (781)932-3959 Facsimile: (781)932-8705 Technical Applications
Sean Gerrin, ALM
14 Gill Street Unit H
Woburn, Massachusetts 01801
(e) sgerrin@covarisinc.com
(p) 781-932-3959 X2300<br>
