Hilley JL et al Sorghum Dw2 encodes a protein kinase regulator of stem internode length Scientific Reports 7 4616 2017 DOI 101038s41598017046095 Objective ID: 1041384
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1. Identification of a stem length regulator in sorghumHilley, J.L. et al. Sorghum Dw2 encodes a protein kinase regulator of stem internode length. Scientific Reports 7, 4616 (2017) [DOI:10.1038/s41598-017-04609-5]. Objective Identify the gene corresponding to the Dw2 locus to better understand how stem growth, a key factor in biomass yield and plant architecture, is controlled in sorghum.ApproachMeasure length of each internode in sorghum genotypes from several RIL populations known to segregate for Dw2/dw2 alleles.Conduct Quantitative Trait Locus (QTL) mapping, fine map the Dw2 locus, and identify candidate genes/alleles in the delimited Dw2 locus.Identify the Dw2 gene sequence and likely biochemical function; conduct phylogenetic analyses, and trace the origin and utilization of Dw2 alleles in sorghum breeding programs. Determine Dw2 expression in various tissues using the sorghum RNA Atlas.Results/ImpactsThe Dw2 gene was identified as a protein kinase whose closest homolog in Arabidopsis is KIPK, a member of the AGCVIII protein kinase family that interacts with KCBP (lipid vesicle transport) and PERK (cell wall signaling). Therefore, Dw2 may regulate stem growth by modulating delivery and utilization of materials for cell wall biosynthesis.Increasing our knowledge of stem growth will aid the improvement of sorghum hybrids for bioenergy production. BRC Science Highlight GLBRC August 2017DYM corresponds to dominant Dw2, whereas DDYM corresponds to the recessive dw2 allele.