PPT-Figure Figure. Three Mollaret-like cells are present (center), with a neutrophil
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Procop GW YenLieberman B Prayson RA Gordon SM Mollaretlike Cells in Patients with West Nile Virus Infection Emerg Infect Dis 2004104753754 httpsdoiorg103201eid1004030783
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Figure Figure. Three Mollaret-like cells are present (center), with a neutrophil: Transcript
Procop GW YenLieberman B Prayson RA Gordon SM Mollaretlike Cells in Patients with West Nile Virus Infection Emerg Infect Dis 2004104753754 httpsdoiorg103201eid1004030783. Ng M, Lee J, Leong M, Ling A, Tan H, Ooi E. Topographic Changes in SARS Coronavirus–infected Cells during Late Stages of Infection. Emerg Infect Dis. 2004;10(11):1907-1914. https://doi.org/10.3201/eid1011.040195. Pantoja CR, Navarro SA, Naranjo J, Lightner DV, Gerba CP. Nonsusceptibility of Primate Cells to Taura Syndrome Virus. Emerg Infect Dis. 2004;10(12):2106-2112. https://doi.org/10.3201/eid1012.040419. van Ginkel FW, Nguyen HH, McGhee JR. Vaccines for Mucosal Immunity to Combat Emerging Infectious Diseases. Emerg Infect Dis. 2000;6(2):123-132. https://doi.org/10.3201/eid0602.000204. Dumler J, Choi K, Garcia-Garcia J, Barat NS, Scorpio DG, Garyu JW, et al. Human Granulocytic Anaplasmosis and Anaplasma phagocytophilum. Emerg Infect Dis. 2005;11(12):1828-1834. https://doi.org/10.3201/eid1112.050898. Yang J, Ramanathan MP, Muthumani K, Choo AY, Jin S, Yu Q, et al. Induction of Inflammation by West Nile virus Capsid through the Caspase-9 Apoptotic Pathway. Emerg Infect Dis. 2002;8(12):1379-1384. https://doi.org/10.3201/eid0812.020224. Goosney DL, Knoechel DG, Finlay BB. Enteropathogenic E. coli, Salmonella, and Shigella: Masters of Host Cell Cytoskeletal Exploitation. Emerg Infect Dis. 1999;5(2):216-223. https://doi.org/10.3201/eid0502.990205. The PMN enrichment was carried out by double gradient centrifugation. A single cell suspension was (1x10^5 cells) stained with the following markers: CD15-BV421 (Pan-granulocyte), CD66b-FITC (Neutrophil maturity), CD16-APC (Neutrophil FC receptor), CCR3-APC-Cy7 (Eosinophil and basophil), and CD56-PerCP (NK cell). PMN population was gated based on the FSC-SSC scatter plot. The CD15+ and CD66b+ double positive cells were gated using a quadrant plot. This population indicated neutrophil enrichment, which was an average of 85.45% (± 6.20%). We also looked at contaminating eosinophil/basophil (CCR3) and NK-cell (CD56) populations by plotting against CD16 (neutrophil specific), and found them to be 1.14% (± 1.02%) and 1.9% (± 2.00 %) respectively. . Ng M, Lee J, Leong M, Ling A, Tan H, Ooi E. Topographic Changes in SARS Coronavirus–infected Cells during Late Stages of Infection. Emerg Infect Dis. 2004;10(11):1907-1914. https://doi.org/10.3201/eid1011.040195. Salzberg SL, Kingsford C, Cattoli G, Spiro DJ, Janies DA, Aly MM, et al. Genome Analysis Linking Recent European and African Influenza (H5N1) Viruses. Emerg Infect Dis. 2007;13(5):713. https://doi.org/10.3201/eid1305.070013. Weissenböck H, Bakonyi T, Sekulin K, Ehrensperger F, Doneley RJ, Dürrwald R, et al. Avian Bornaviruses in Psittacine Birds from Europe and Australia with Proventricular Dilatation Disease. Emerg Infect Dis. 2009;15(9):1453-1459. https://doi.org/10.3201/eid1509.090353. Wolfs TF, Wagenaar JA, Niesters H, Osterhaus A. Rat-to-Human Transmission of Cowpox Infection. Emerg Infect Dis. 2002;8(12):1495-1496. https://doi.org/10.3201/eid0812.020089. Keawcharoen J, van Riel D, van Amerongen G, Bestebroer TM, Beyer WE, van Lavieren R, et al. Wild Ducks as Long-Distance Vectors of Highly Pathogenic Avian Influenza Virus (H5N1). Emerg Infect Dis. 2008;14(4):600-607. https://doi.org/10.3201/eid1404.071016. Lee J, Chung I, Shin D, Cho S, Cho D, Ryang D, et al. Hemorrhagic Fever with Renal Syndrome Presenting with Hemophagocytic Lymphohistiocytosis. Emerg Infect Dis. 2002;8(2):209-210. https://doi.org/10.3201/eid0802.010299. Dunkle SE, Mba-Jonas A, Loharikar A, Fouché B, Peck M, Ayers T, et al. Epidemic Cholera in a Crowded Urban Environment, Port-au-Prince, Haiti. Emerg Infect Dis. 2011;17(11):2143-2146. https://doi.org/10.3201/eid1711.110772.
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