PPT-Biology 227: Methods in Modern Microscopy

Andres Collazo Director Biological Imaging Facility Steven Wilbert Graduate Student TA Week 4 Live imaging zebrafish embryos George Streisinger developed zebrafish as a model system From Viruses to Zebrafish. Microlens. Array. 18 October, . FiO. 2011. Antony Orth and Kenneth . Crozier. High Throughput Microscopy. 1. http://www.olympus.co.uk/microscopy/22_scan_R.htm#. High throughput fluorescence imaging by scanning sample under . Adrian Pieri, Tom Nowak and Olivia Hughes. Introduction. The project that we undertook was about the exploration and analysis of various methods such as laser diffraction, optical microscopy and Atomic Force Microscopy in order to measure the width of a single strand of human hair using concepts of nanotechnology and microscopy.. Chelsea Aitken. Peter Aspinall. Advantages Over Light Microscopy. Resolution of light microscopy limited by Rayleigh Criterion. If two objects cannot be seen as distinct structures, then they may be considered coincident in space. Lecture 13:. Super-resolution microscopy: Part I. Lecture 13: . Fluorescent labeling, multi-. sprectral. imaging and FRET. Review of previous lecture. FRET. FLIM. Super resolution . microscopy. NSOM. Lecture 18:. High speed microscopy, Part 2. High speed microscopy, Part . 2: Spatial . light modulator microscope and other 3D sensors. Making laser scanning confocal microscopes faster. Resonant scanner confocal. Fire Protection Laboratory Methods Day. June 25, 2014. Paul M. Anderson. Graduate Research Assistant. University of Maryland. Department of Fire Protection Engineering. Transmission Electron Microscopy. Lecture . 08: . Contrast and Resolution. Lecture 8: Contrast and Resolution. Bright-field. Tinctorial. dyes: the first contrast. Review . of Kohler Illumination. Tradeoffs . in Contrast/Resolution. Dark . II. MENA3100,OBK, . 29.01.15. We don’t read all. You don’ have to read all. 1.3 Specimen Preparation. Read it. 1.4.1 Bright-Field and Dark-Field. Definetly. 1.4.2 Phase-Contrast. Cursori. 1.4.3 Polarized-Light. Lecture 16:. Super-resolution microscopy: Part . 2. Lecture 16: Super-resolution microscopy and TIRFM. Single molecule imaging. Total internal reflection fluorescence microscopy (TIRFM). Super-resolution techniques . Jenny . Malmstrom. AFM invented by Binning and co-workers in 1986. . Belongs to the Scanning Probe Microscopy family. Binning et al., Physics Review Letters 1986. AFM invented by Binning and co-workers in 1986. . Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Andres Collazo, Director Biological Imaging Facility. Wan-. Rong. (Sandy) Wong, Graduate Student, TA. Lecture . 7: Confocal Microscopy. Microscopy is the technical field using microscopes to view samples and objects that can not be seen without unaided eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron and scanning probe microscopy.. KAARTHIK J. INTRODUCTION. A fluorescence microscope is a optical microscope that uses fluorescence and phosphorescence instead of , or I addition to reflection and absorption to study properties of organic or inorganic substances.. (SEM) Electron Microscopy (SEM) and TEM Scanning electron microscopy is used for inspecting topographies of specimens at very high magnifications using a piece of equipment called the scanning electr