PPT-Biology 227: Methods in Modern Microscopy

Andres Collazo Director Biological Imaging Facility Steven Wilbert Graduate Student TA Week 4 Live imaging zebrafish embryos George Streisinger developed zebrafish as a model system From Viruses to Zebrafish. Lecture . 09: . Polarization and DIC. Lecture 9: Polarization and DIC. Review Contrast and Phase Contrast. Polarization. Birefringence. Nomarski. . (Differential . Interference Contrast). Resolution. Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Lecture . 7: Confocal Microscopy. Optical Sectioning: adding the third dimension. Wide-field . Imaging. Point . Spread Function. Deconvolution. Lecture 13:. Super-resolution microscopy: Part I. Lecture 13: . Fluorescent labeling, multi-. sprectral. imaging and FRET. Review of previous lecture. FRET. FLIM. Super resolution . microscopy. NSOM. Lecture 06: . Fluorescence Microscopy. Lecture . 6: Fluorescence . Microscopy. Detectors for Microscopy, Part 2. CMOS, PMT and APD. Phenomenon . of Fluorescence. Energy . Diagram. Rates . of excitation, emission, ISC. Lecture 18:. High speed microscopy, Part 2. High speed microscopy, Part . 2: Spatial . light modulator microscope and other 3D sensors. Making laser scanning confocal microscopes faster. Resonant scanner confocal. Lecture 05: . Illumination and Detectors. Lecture 5: Illumination and Detectors. Review diffraction. Illumination sources. Tungsten-Halogen. Mercury arc lamp. Metal Halide Arc lamps. Xenon Arc lamps. Writing scientific papers. . Understanding how to do science is a powerful insight . Communicating science is critical to success and progress in science. Good writing comes from clear thinking. Precision in writing (language) is critical to communication. Lecture . 08: . Contrast and Resolution. Lecture 8: Contrast and Resolution. Bright-field. Tinctorial. dyes: the first contrast. Review . of Kohler Illumination. Tradeoffs . in Contrast/Resolution. Dark . Eric Abdullayev . MD. , MBA,CEBT. International Sight Restoration Eye Bank (ISR), Tampa FL, . USA . Author Has No Financial Interest To Disclose. Purpose: . To introduce a method of telemedicine for performing corneal . Lecture 16:. Super-resolution microscopy: Part . 2. Lecture 16: Super-resolution microscopy and TIRFM. Single molecule imaging. Total internal reflection fluorescence microscopy (TIRFM). Super-resolution techniques . Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Andres Collazo, Director Biological Imaging Facility. Wan-. Rong. (Sandy) Wong, Graduate Student, TA. Lecture . 7: Confocal Microscopy. Replica of van 1670 Moody Use the information in this tutorial to supplement the visuals in lab and the Chapters 1 8 and 9 in your lab manual Replica of Culpepper tripod microscope built c 1725 by Co The physical background of medical . tomographies. Lecture. 7. Modern imaging techniques in biology: Lecture 7. 1. MRI. . thematics. Microscopic and macroscopic magnetization. The Bloch equation. T. Type into your browser’s address bar: . www.tbrieder.org. In the navigation panel click:. Presentations. Bacteriologic Basis of Tuberculosis Control. Base . bactériologique. de la . lutte. . contre.