Mark Windham University of Tennessee David Byrne Texas AampM University What has been accomplished Tools developedadapted to rose Diagnostic tools virus Molecular Immunological ID: 931249
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Slide1
Future Directions in RRD Research
Mark Windham – University of Tennessee
David Byrne – Texas A&M University
Slide2What has been accomplished?Tools developed/adapted to rose
Diagnostic tools – virus
Molecular
Immunological
Diagnostic tools – mite
Molecular
Visual (electron microscopy)
Sampling protocol for virus (still needs work)
Sampling tools for mite
Plant tissue
Air sampling
Slide3What has been accomplished?Tools developed/adapted for rose
Monitoring app on web site
Phenotyping protocol
Genotyping protocol
GBS and map for diploid
Populations for genetic studies
Diploid and tetraploid
Analytical tools –
FlexQTL
for diploid
Slide4What has been accomplished?Descriptive research
Virus sequence
Genetic variability of the virus
Identify the vector/other mites on roses
Identify mite predators associated with rose
Mite behavior on rose – feeding and transmission
Mite distribution
Within plant
Geographically
Slide5What has been accomplished?Descriptive research
Miticide efficacy
Pruning and virus elimination
Garden design and mite movement
Symptomology
over roses
Sources of rose rosette
resistance
Genotypic map of rose
Slide6Rose Rosette Virus
Rose
Phyllocoptes
mite
Rose Rosette Disease
Host reaction
Host range
Transmission efficiency
Vector specificity
Ecology and Climate
Genotype and phenotype
Feeding behavior
Slide7Distribution of RRD in the USA
Slide8What do we need to do?
Virus biology
Mite biology
Host plant resistance
Slide9Virus Biology
Slide10Virus biology?Mechanism of resistanceVirus effect on mite (Tzanetakis research)
Virus movement/distribution in plant
Virus variation in virulence
Sampling protocol for virus
Symptom development
Early detection – remote sensing
Multispectral
Raman spectrometer
Slide11Mite Biology
Slide12Mite BiologyMite rearing and developmentMite monitoring
Mite epidemiology
Life cycle studies
Environmental influences
Preference/biology on resistant roses
Mite distribution on plant/in field
Survey of mite species in USA
Mite transmission
Slide13Breeding and Resistance
Slide14Breeding and ResistanceMore efficient screening techniqueEffect of resistant rootstock on scion
Mechanism of resistance
Inheritance of resistance
Marker development
Marker validation
Marker shared with private breeders
Slide15Constructing Management Strategies
Slide16Slide17Slide18Marketing and Economics
Slide19Slide20Slide21Slide22