Iodine Test Polysaccharides Polysaccharides are large carbohydrate that consist of many monosaccharaides linked by glycoside bonds Poly saccharides have two main functions in organisms ID: 934801
Download Presentation The PPT/PDF document "Determination of polysaccharides" is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Slide1
Determination of polysaccharides
(Iodine Test)
Slide2Polysaccharides
Polysaccharides
are large
carbohydrate that consist of many
monosaccharaides
linked by
glycoside
bonds
.
Poly saccharides have two main functions in organisms:
➢Storage of energy.
➢Structure and
protection
Example of Polysaccharides such as (glycogen ,cellulose , starch)
Slide3Classification
ofpolysaccharides
Slide4Structure of Polysaccharides
Slide5Important consideration
Glycoside bonds
: also called
glycosidic linkage
is a type of covalent
bond
that joins a carbohydrate (sugar) molecule to another group, which may or may not be another
carbohydrate.α-Amylase: is an enzyme that produced by salivary gland in the mouth and by
pancreas which breaks down starch in order to be ingested by the body.
Human cannot break down ß-1-4 glycosidic bond in cellulose, that’s why it’s difficult to hydrolyze this complicated long chain of sugar inside of cellulose.
Slide6Why that body use carbohydrates as source of energy
1-
If the body cells require instant energy (as in exercise), the glucose is supplied with oxygen from the blood to provide energy
.
2-
If our body does not need immediate energy the sugars and glucose are converted into a long chain molecule called glycogen in the liver for storage.
3- As term like
Glucose
it can also be used by the cells to synthesize amino acids, which in turn can be linked up to form proteins and are stored.
4- If all the storage areas of glucose are full, it is used to make fats which can be stored in many places all over the body.
Slide7Iodine Test
Aim of the test is for determination of polysaccharides and indicating them among other saccharides like monosaccharide and disaccharides .
Slide8Principle
Iodine - KI Reagent: Iodine is not very soluble in water, therefore the iodine reagent is made by dissolving iodine in water in the presence of potassium iodide.
This
makes a linear
triiodide
ion complex with is soluble. The
triiodide
ion ion slips into the coil of the starch causing an intense blue-black color.
Slide9Procedure
Prepare Iodine solution
Prepare sample like , Flour , Starch , Glucose , Sucrose
Add 1 ml of each of the samples into labeled test tubes.
Drop 3 drop of iodine solution to each test tubes .
Put test tubes into a beaker and leave it inside water bath for 15 mint
Results will be visualized by color detection .
Slide10Observation