1675 University Blvd WEBB 416 amp 418 Lab phone205 9962661 Director Douglas Moellering PhD Research Associates Kelley SmithJohnston BS johnkuabedu Melissa J Sammy PhD mjsammyuabedu ID: 931449
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Bio-Analytical Redox Biology (BARB) Core
1675 University Blvd, WEBB 416 & 418Lab phone:(205) 996-2661Director: Douglas Moellering, PhDResearch Associates: Kelley Smith-Johnston, BS: johnk@uab.eduMelissa J. Sammy, PhD: mjsammy@uab.edu
BARB
Volker Hall
WEBB
16
th
St.
18
th
St.
8th Ave
Slide2Mitochondrial Bioenergetics
High Resolution Respirometry
Seahorse Extracellular Flux
In permeabilized tissue and/or mitochondria isolated from:
Slide3Mitochondrial Electron Transport Chain Kinetics
Samples:Frozen tissueIsolated MitochondriaTotal Homogenates
Cortisol Assay
(long-term stress)
Samples: Hair and Nails
Slide4Mitochondrial DNA (mtDNA) Assays
mtDNA ContentmtDNA Damage
mtDNA
DAMPs
Redox Assays
Glutathione (GSSG and GSH)
Samples: serum/plasmaSamples: frozen tissues, cells, buffy coat
Samples: frozen tissues, cells, buffy coat
Glutathione Peroxidase (GPX)
Superoxide Dismutase (SOD)
Catalase
Slide5Bio-Analytical Redox Biology (BARB)
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