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Cardiopulmonary  History and Exam Cardiopulmonary  History and Exam

Cardiopulmonary History and Exam - PowerPoint Presentation

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Cardiopulmonary History and Exam - PPT Presentation

Wendy Blount DVM Housekeeping Dylan is our concierge Let her know if you need another note pad Or anything else Course materials are also downloadable at httpwendyblountcom Direct link ID: 931654

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Slide1

Cardiopulmonary History and Exam

Wendy Blount, DVM

Slide2

Housekeeping

Dylan

is our “concierge”Let her know if you need another note pad

Or anything else!Course materials are also downloadable at

http://wendyblount.com

Direct link:

http://

wendyblount.com/cardiology.php

Click on Presentation Notes

Slide3

Dylan is our “concierge”

Let her know if you need another note pad

Or anything else!

Course materials are also downloadable at

http://wendyblount.com

Direct link:

http://wendyblount.com/cardiology.php

Click on Presentation Notes

Housekeeping

Slide4

Housekeeping

Click on this presentation at the top

Slide5

Click on this presentation at the top

Housekeeping

Slide6

Seminar Packet:

Flash DriveAgenda

Evaluation

CE Certificatepen and notepad

List of AbbreviationsHank and Eli Fund, Dark Horse Lodge info

Cardiology Form, GlobalFAST® Forms & Handouts

Instructions for screen sharing

Info on hands-on ultrasound training

Housekeeping

Slide7

F

lash Drive:

All PowerPoints with embedded videos

.pdfs of PowerPoints – 1 and 6 slides per page

Forms – in clinic and lab submission

Scientific articles

Diagnostic and treatment aid handouts

Agenda, abbreviations, instructions for following along

Hank and Eli Fund, Dark Horse Lodge info

Housekeeping

Slide8

We will take a 5 minute break each hour and a

half, but take your own breaks as you wish

PLEASE PARTICIPATE!!!

But take private conversations outside or, in the classroom or store

Get

your CE certificate signed at the end of each day

If you leave early, the time will be added to the form

Pretty Please fill out the Course Evaluation form

Leave with DylanHousekeeping

Slide9

Smoking outside the entrance, in the designated area

Restrooms through the door at the back of the room, and to the left of the classroom

Lunch will be served there There is no cell service in the building – enable WiFi Calling

WiFi: MeadowRidge Password: safety01

Our Host

Slide10

IR (non-contact) temperature at morning sign in

Anyone with a fever will not be admitted

Tables & chairs disinfected with Rescue dailyRescue wipes and spray at the sanitation table

Treat it like a surgery tableUse hand sanitizer each time you access the table

Masks are encouraged but not requiredIf you come within 6 feet of another person, please wear your mask – especially during breaks, meals and the dry lab, and especially for people >65yrs

Social Distancing

Slide11

Those who are

already significantly exposed to each other prior to the seminar may

share a tableNo more than 2 people at a table

The seat you have chosen is yours for the weekend Place

OCCUPIED table marker in your packet on your table, so others know it is reserved for you.

Wear the

HIGH RISK nametag

in your packet if you wish

Hand sanitizer at your table – use it oftenWash your hands as often as is possibleNo touching anyone else’s table or chair or ANYTHING YOU CAN AVOID TOUCHING!Social Distancing

Slide12

When lining up for food or dry lab, please stay 6 feet behind the next person

MASKS ARE REQUIRED FOR THE DRY LABFood will be served to you, and single use condiments are available on request

Sanitize hands before (and after if you can)touching any doorknobs or other shared items

Touching the sign-in table – Dylan will sign you in and I will sign you out

Touching anything but your stuff on your table

Social Distancing

Slide13

Screen at the front of the roomFlash Drive

.pdfs of PowerPoints - 1 & 6 slides/page.pptx of PowerPointsFiled in section folders

Website – updated PowerPointshttp://wendyblount.com/cardiogy.php

Screen Sharing: www.startmeeting.com

Click on the “join” button at the top rightFollowing Along

Slide14

Following Along

Screen at the front of the room

Flash Drive

.pdfs of PowerPoints - 1 & 6 slides/page

.pptx of PowerPoints

Filed in section folders

Website – updated PowerPoints

http://wendyblount.com/cardiogy.php

Screen Sharing: www.startmeeting.comClick on the “join” button at the top right

Slide15

Screen at the front of the room

Flash Drive

.pdfs of PowerPoints - 1 & 6 slides/page

.pptx of PowerPoints

Filed in section folders

Website – updated PowerPoints

http://wendyblount.com/cardiogy.php

Screen Sharing: www.startmeeting.comClick on the “join” button at the top right

Following Along

Slide16

Screen at the front of the room

Flash Drive

.pdfs of PowerPoints - 1 & 6 slides/page

.pptx of PowerPoints

Filed in section folders

Website – updated PowerPoints

http://wendyblount.com/cardiogy.php

Screen Sharing: www.startmeeting.comClick on the “join” button at the top right

Following Along

Slide17

Screen at the front of the roomFlash Drive

.pdfs of PowerPoints - 1 & 6 slides/page.pptx of PowerPointsFiled in section folders & PowerPoint folder

Website – updated PowerPointshttp://wendyblount.com/neurology.php

Screen Sharing: www.startmeeting.comClick on the “join” button at the top right

Following Along

Slide18

As referral medicine becomes more advanced, it by default becomes more expensive

There is a growing gap between general practice and specialty practices

These seminars intend to fill that gapEverything we talk about this weekend can be done in a rural mixed animal practice with no emergency clinic or specialists within a few hours

DISCLAIMER – I AM NOT A SPECIALIST

Practical VetMed Philosophy

Slide19

Our Goal for the

Weekend:Review

common things in detailCover

uncommon things in less detailRecognize

them and refer to the proceedings

Increase

level of care at your

clinic

And/or be better referring vetsBuild relationships with your colleaguesTexasVets list – Rosemary Lindsey, moderator

rosemarylindsey@sbcglobal.net

Practical VetMed Philosophy

Slide20

Hank & Eli’s

Fund is

a

source of financial support

for active

duty service

men and women,

retired veterans’ service animals, and retired military animals

with veterinary medical

bills

There is a one page flyer

in your packet

Letter from Colton’s mother

on flash drive

Donation box on Dylan’s desk

give online

:

http://www.hankandeli.com

Mail donations to address on flyer

Free PowerPoint Templates

Hank & Eli’s Fund – Dark Horse Lodge

$3302

so far…

Slide21

Free PowerPoint Templates

Hank & Eli’s

Fund

Slide22

Free PowerPoint Templates

Hank & Eli’s

Fund

Southside Animal Hospital

6940 S Padre Island Dr

Corpus Christi TX 78412

(361) 993-7388

mmooremailforme@yahoo.com

If you want a collection box for your clinic,

contact Dr. Mike Moore

Slide23

Slide24

Signalment

Age

Congenital disease young

Myxomatous Valvular Diseaseold

ExceptionsCavalier King Charles Spaniels

mild PDA, PS, SAS

Reverse PDA

HCM in purebred cats

Slide25

Signalment

Breed

Boston TerrierCavalierCocker Spaniel

Boxer Doberman

English BulldogGolden Retriever

HBT,

ColTr

C

VDDCM, PS, PDA, 3rdAVHBT, PS, SAS

DCM, Boxer CM, ASD

DCM (Arrhythmia?)

SAS, PS, CVDSAS

Slide26

Signalment

Breed

Great DaneGSDIrish Setter

Irish WolfhoundKeeshondLabrador

Maine CoonNewfoundland

DCM, CVD

PRAA, SAS, PDA

PRAA

DCMToF (define), MVDTVD

HCMDCM, SAS

Slide27

Signalment

Breed

Persian/HimalayanPointerPoodle

St BernardSamoyedSchnauzer

Springer SpanielYorkie

HCM

PRAA, SAS

CVD, PDA, CB

DCMASD, PSSSS, CVD, PS, CBVSD

CVD, CB, CT

Slide28

History - Collapse

How can you tell the difference between seizure and syncope?

Urination/defecation/vocalization/

paddlingStiff/opisthotonus or flaccid

Narcolepsy, exercise induced collapseTwitching and muscle fasciculations

Cyanosis, pallor

Abnormal behavior before and after

Duration of stiffness/opisthotonus

Many times, you can’t (especially when short)

Slide29

History - Collapse

What causes syncope?

BradyarrhythmiaPeriod of asystole

TachyarrhythmiaObstruction of blood flow to or from the heart

Inability to deliver oxygen to the brain, especially when there is increased demand

Decreased CO - Heart Failure**

Lung/airway disease

Anemia or other RBC/Hb problem

Slide30

History - Collapse

What causes syncope?

Bradyarrhythmia3rd

degree heart block (define)

Sick sinus syndrome

(define)

Period of asystole

Sick sinus syndrome (SSS)Vagal surge (examples)Abdominal dz & retchingIntubation (brachycephalic)

Slide31

History - Collapse

What causes syncope?

Tachyarrhythmia burst

Vtach (define, causes)B

oxer C

ardio

M

yopathy

Myocarditis (Chagas, Parvovirus)Myocardial hypoxiaAbdominal pathology (spleen)Supraventricular tachycardia (SVT) (define)Re-entry pathway (define)

Atrial fibrillation (Afib)SSS

Slide32

History - Collapse

What causes syncope?

Obstruction of a great vessel or heart chamberThrombus (clot or HWDz)

NeoplasiaExtramural mass

Increased oxygen demand can not be met due to severe cardiovascular or pulmonary disease

AKA Exercise intolerance

Slide33

History - Cough

How can you tell the difference between cardiac and respiratory cough/dyspnea?

Honking cough, soft moist cough, dry hacking cough

Coughing/gagging up white foamy fluid, acting like something caught in the throat

Coughing up blood tinged fluidCough when drinking water, on tracheal palpation, or exercise induced cough

Presence of a murmur

(big dog, little dog, cat)

Many times, you can’t without PE/diagnostics

Slide34

History - Cough

Cough on tracheal palpation

Any dog or cat will cough a few times on vigorous tracheal palpationProlonged coughing after tracheal palpation often indicates pathology (cardio or resp?)

equally likely with airway disease and cardiovascular disease

Slide35

History - Cough

Dogs vs Cats

Coughing cats

much more likely to have respiratory disease than heart failureCats with heart failure more often present with acute and severe dyspnea, with no cough

Some cat owners can find it difficult to distinguish vomiting, gagging and coughingCoughing dogs can have either or both

Slide36

Exam – Stethoscopes

Ear pieces fit snugly in the ears

Angle fits your ear canalsPoor fit, and you’ll miss low intensity murmurs

Tubing longer than 18 inches will dampen sounds

Electronic stethoscopes (microphone based)Difficult to distinguish heart from lung sounds

Difficult to distinguish patient from background noise

Meditron

sensor based scope

eliminates problemsConnect to computer & record for PCG consult

Slide37

Exam – Stethoscopes

Pediatric stethoscope (

infant & pediatric sizes

)For cats and small dogs

Will distort and decrease sound intensity if used on a medium or large dog

Adult stethoscope

For medium to large dogs

Won’t localize murmurs properly in cats and small dogs

Slide38

Exam – Stethoscopes

Cardiology Stethoscope - Diaphragm

Filters out low frequency sounds to hear high frequency sounds better

Press firmly against the chest

BellFor low frequency sounds (S3 S4 in dogs)

Press gently against the chest

Slide39

Auscultation

Minimizing patient noise

Panting, whining – close mouth, occlude nostrilsPurring ( audio

)Aversives – turn water on, show another animal

Gentle pressure on the larynxCotton ball with alcohol to the noseJiggle the doorknob

Sometimes sedation is needed (

chart

)

Acepromazine 0.0125-0.025 mg/lb, maximum 1 mg per dogButorphanol 0.1 mg/lb or buprenorphine 0.01-0.02 mg/kgIV the fastest and most profound (30-45 minutes)

Slide40

Auscultation

Patient is standing in a quiet place

R Lateral recumbency and listen from bottom if muffledFirm pressure with the diaphragm to avoid hair noisesget comfortable ausculting heart and palpating pulses at the same time

Listen at least 5 min for cardiac patientsHeart - R and L apex, L bases

L armpit (30 sec+ each)

Lungs – RCr, RMid,

RDCd,

LCr, LMid, LDCd

(20 sec+ each)

Slide41

Auscultation

Is the murmur hemodynamically significant?

Prolonged and loud - yes

Pansystolic - yes

Diastolic - yes

Low intensity

- maybe

Early systolic

- maybeMusical – maybe not so muchLoudness is not necessarily correlated to presence of heart failure

Slide42

Auscultation – Lung Sounds

Snaps crackles and wheezes

(cardio or resp?)More likely respiratory in dogs

(audio)

Not very sensitive for pulmonary edemaBeware similar hair rubbing noises

Pleural/pericardial Rubs

(

pleural rubs) (pericardial rubs)Dull/absent lung sounds (dog vs cat) (causes)Lung consolidationPneumothorax, pleural effusion (TFAST®

)Harsh lung sounds with no murmur in cat

think asthma or heartworm disease (

audio)

Slide43

Auscultation - Heart Sounds

Normal Heart Sounds

Slide44

Auscultation - Heart Sounds

Normal Heart Sounds

S1

AV Valves closeBeginning of systole/End of diastoleS2

Semilunar valves closebeginning of diastole/End of systole

Tachycardia – which is which?

S2 shorter and higher frequency

(

audio)Pulse is during systole

Slide45

Auscultation - Heart Sounds

Variable intensity S1

Arrhythmia (variable FS)

Louder S1 (AV slamming)

Young, narrow chested dogs (Doberman

)

Increased sympathetic tone

Anemia

(decreased blood viscosity)FeverHypertensionAdvanced mitral valve disease

Slide46

Auscultation - Heart Sounds

Quieter S1

(AV softly closing or muffled)Obesity, barrel chested dogs

Myocardial failure (decreased FS)

Pronounced 1st degree heart block

Hypervolemia

(IV fluids, hypernatremia, LHF)

Slide47

Auscultation - Heart Sounds

Louder S2

(SL slamming)Hyperthyroidism

Fever, anemiaHeartworm DiseaseCor pulmonale

(define)Quieter S1-S2

(SL softly closing)

Myocardial failure (DCM, severe MR)

Obesity, barrel chested

Slide48

Auscultation - Heart Sounds

Third Heart Sound (Gallop)

S3 (S1-S2-S3)

S4 (S4-S1-S2)Split S2Systolic Click

Summation Gallop (S4-S1-S2-S3)

Slide49

Auscultation - Heart Sounds

Third Heart Sound

S3 – protodiastolic gallop (S1-S2-S3)

Rapid LV filling – early diastole (audio

)PMI R or L apex – low frequency (best heard with the bell)

At maximal mitral opening (E point on echo)

stiff LV or large diastolic volume

HCM, RCM, DCM, severe MR

Indicates myocardial failureUsually a bad mamma jamma

Slide50

Auscultation - Heart Sounds

Third Heart Sound

S4 – presystolic gallop (S4-S1-S2)Atrial contraction -

Late diastole (audio)

PMI R or L apex, low frequency (bell)Stiff LV (HCM)

Increased afterload

3

rd

degree AV blockMyocardial failure (DCM, bad MR)Sometimes heard in normal cats & giant dogsnot necessarily a bad mamma jamma

Slide51

Auscultation - Heart Sounds

Third Heart Sound

Split S2

PMI right heart base (left side)

AoV PV don’t close at same time (PV later)Reverse PDA

Pulmonary hypertension (HWDz, COPD)

Severe RBBB

relative PS of right to left shunts (ASD)

normal variation in large dogs (audio)

Slide52

Auscultation - Heart Sounds

Third Heart Sound

Systolic Click

Very sharp, high frequency click of Mitral valve prolapse, in early MVD

Snapping of the chordae tendinae as they go taughtPMI left apex

Mid-Systolic (

audio

)

May be accompanied by a systolic murmurEarly, late, or holosystolic (audio)Often years until CHF develops, if at all

Slide53

3 Heart Sounds

How Can you tell the difference?

Does it Matter?Systolic less likely pathogenic (

S1-S2-S3)Systolic Click sounds sharper

Diastolic more likely pathogenic (

PMI L base)

(

S4-S3-S1

)How Can you tell if S3 or S4?Can’t tell if heart rate is > 160-180 (summation)just do a cardio work-up

Slide54

1 – left apex (MV)2 – left base (AoV)

3 – right base (PV) – ausculted on the left

4 – right apex (TV)

5 – left armpit (PDA)

5

Auscultation –

Heart Sounds

PMI

leftright

Slide55

Auscultation – Heart Sounds

PMI (Point of Maximal Intensity)

Left Apex – at palpable apical bea

t S1 - MR (

mild MR) (

severe MR

)

Left Base –

slide cranially & a little dorsallyS2 - SAS (audio)S1, S2 - Ao endocarditis (audio

)Right Base (left side)

S2 - PS (

mild PS) (severe PS

)

Left Axilla

continuous - PDA (

PDA

) (

severe PDA

)

Right Apex

S1 – TR – like MR but often quieter (

audio

)

Slide56

Auscultation – Heart Sounds

Muffled Heart Sounds

(causes)

Pleural, Pericardial effusion (*difference*)

Diaphragmatic hernia, thoracic massesObesity

What besides cardiac disease can cause a pathologic murmur?

Anemia

hypoproteinemia

Slide57

Auscultation – Heart Sounds

Why do puppies have innocent murmurs?

Musical, grade 1-2, short

(audio

) (mild MR)

Larger SV relative to great vessel size

Lower PCV and plasma proteins

Artifact – high frequency breath sounds

They tend to be musical and relatively quiet

Slide58

Auscultation – Heart Sounds

Why do cats have innocent murmurs?

Short systolic murmur at the sternum (

audio)

Not pansystolic and usually not holosystolicSympathetic tone

episodic hypertension, increased SV

Can come and go during a vet visit

Slide59

Auscultation – Murmur Grade

Grade 1

Heard in a very quiet room, concentrating

Grade 2easily heard on the PMI - focal

Grade 3

Moderately loud

Grade 4

Very loud over much of the chest

Grade 5Heard with edge of stethoscope on chest, palpable thrillGrade 6Heard with stethoscope off chest, palpable thrill

Slide60

Auscultation – Murmur Grade

High grade murmurs are more likely to be associated with severe disease

Severe disease can also be present with low grade murmur

Occasionally no auscultable murmur in the cat (dog?)DCM

ASDVSD

Reverse PDA (right to left shunting)

Dogs almost never have CHF without a murmur

Cats can have CHF without a murmur

Slide61

Auscultation – Lung Sounds

6 points

3 Right, 3 Left – correspond to lung lobes

Slide62

Auscultation – Murmurs

Holosystolic –

you can still make out S1 & S2

Starts at the end of audible S1Ends at the start of audible S2Murmur between the heart sounds

( VSD

)

Slide63

Auscultation – Murmurs

P

ansystolicStarts before/at the beginning of S1

Ends at/after the end of S2Just hear the murmur with no distinct HS

( severe MR

)

(

severe SAS

)( severe PS )

Slide64

Respiratory Sinus Arrhythmia

Heart rate increases during respiration

Due to increased vagal toneNormal variation in dogs (

not cats)No pulse deficits

If present, heart failure is not likelyIncreased sympathetic tone overrides

Pronounced in disease processes of increased vagal tone

Increased CSF pressure

Chronic respiratory disease

Thoracic or abdominal diseaseAfter sedation

Slide65

Respiratory Sinus Arrhythmia

DDx

Afib with a normal ventricular rateFrequent APCs or VPCs (maybe pulse deficits)

Intermittent SSS

None of these vary consistently with the respiratory cycleRSA is regularly irregular

Others are usually irregularly irregular

(

RSA

) (PS with RSA)

Slide66

Physical Exam – Ascites

most common cause of cardiogenic ascites in cats

TVD > VSD

Tap and do fluid analysis to distinguish between transudate, modified transudate and exudate

(handout)

Usually accumulates slowly, though owners often don’t notice until huge

If truly does develop over days, think

pericardial tamponade

or caval syndrome

Slide67

Exam – Mucous Membranes

Cyanosis

> 4 g/dL of deoxygenated Hb in the blood

Severely anemic animals don’t turn blueEven with life threatening hypoxia

Differential cyanosis (define)

Front of body pink, back of body blue

Reverse PDA, FATE

(why rPDA)

Compare pulse oximetry or blood gases from front of body with rear of bodyWeak or no femoral pulses, pain, paresis with FATEUSE YOUR COLOR DOPPLER!!!

Slide68

Exam – Pulses

Technique

Occlude the pulseThen slowly release pressure until maximum pulse is detected

Pulse Pressure = Systolic – Diastolic

Femoral pulse usually not palpable when MAP <50mmHgDorsal pedal pulse not palpable when SAP <80mmHg

Slide69

Exam – Pulses

Bounding Pulses (water hammer)

Increased systolic pressure (increased SV) (causes)

Aortic regurgitationSevere bradycardia

Thyrotoxicosis (define EF, FS)

Fever

Anemia/hypoproteinemia

decreased diastolic pressure (diastolic runoff)

PDAAV fistulaAortic regurgitation (most common cause)Aortic endocarditis > SAS

Slide70

Exam – Pulses

Weak Pulses

Severely decreased SV – severe forward LHF

Acutely decreased SV – hypovolemiaDecreased peripheral vascular resistance (shock)

Decreased arterial compliance (hypertension)

Pulse peaks slowly and late in systole (

feel the squirt

)

Pulsus parvus et tardus (cause)Severe SAS

Slide71

Exam – Pulses

Short, Brisk Pulses (snappy)

Short, fast systole

Compensated MR (what happens to FS with MR)

Pulse weak or absent during inspiration

Pulsus paradoxus

Systolic pressure falls during inspiration

With pronounced respiratory sinus arrhythmia

Exaggerated by pericardial effusion

Slide72

Exam – Pulses

Alternating Weak and Normal Pulses

Pulsus alternans

Severe myocardial failure (define MF vs CHF) (causes)DCM

RCM, UCM (define)

End stage valvular disease

Prolonged tachyarrhythmia or tachycardia

Slide73

Exam – Pulses

Pulse Deficits (heart beat generates no pulse)

VPCs

Atrial fibrillation with VPCsTachyarrhythmia (inadequate filling)

Every other heart beat has a pulse deficitPulsus bigeminis

Caused by ventricular bigeminy

(define)

Totally chaotic heart sounds and pulses

(audio)Lots of multiform VPCs, Atrial fibrillationDelirium cordis –

like tennis shoes in a dryer

Slide74

Exam – Jugular Veins

Clip or wet the fur over the jugular veins

Evaluate sitting or standing (not sternal)

Jugular Distension (causes)

suggests increased RA pressure (normal dogs cats?)

2-3 cm H

2

0 in cats, 5-8 cm H

20 in dogsOr less often jugular or caval occlusionJugular Pulse (normal dogs cats)5-8cm dorsal to RA in dogs, 2-3 cm in cats (1/3 way up)

Too high indicates increased right heart pressure

If abnormalities above not noted, occlude at thoracic inlet, and releaseHepatojugular reflux

Slide75

Exam – Jugular Veins

Jugular distension, high pulse, +HJR

(causes)Jugular/caval occlusion

Heartworm diseaseExternal mass (cyst, abscess, granuloma, neoplasia)

Thrombus (causes)

Decreased RV compliance

RV hypertrophy

PS, TOF, pulmonary hypertension

Restrictive CMRVOT obstructionHeartworm disease, neoplasia, thrombus

Slide76

Exam – Jugular Veins

Jugular distension, high pulse, +HJR

RV volume overload

TR with RHFVSD

HWDzCompression on the RV, so it can’t fill

Pericardial effusion

constrictive pericarditis

Pericardial mass

Evaluation of CdVC, hepatic & splenic veins on US are more sensitive for increased RV pressure than jugular vein exam

Slide77

Exam – Extremities

Peripheral edema

rareOften accompanied by diarrhea

Due to RHF, including end stage biventricular failure

Cold extremitiesDue to RHF and venous stasis

Or saddle thrombus

Acutely painful, followed by lack of pain

( Cardiovascular Exam form )

Slide78

Summary

PowerPoints

- .pptx,

.pdfs – 1 or

6 slides per page

Form

– Cardiovascular Exam

.docx

, .pdfVet HandoutsSedation Doses and Echo Values by Weight

Fluid Analysis Diagnostic Chart

Audio Files

(29) – thoracic auscultation

Slide79

Summary

Hidden Slides

List of common cardiovascular diseases by breed

More Details on gallop murmursQuiet heart sounds

Slide80

Acknowledgements

Smith FWK, Keene BW, Tilley LP

Rapid Interpretation of Heart and Lung Sounds, 2nd ed, 2006

Kvart C & Haggstrom J

Cardiac Auscultation and Phonocardiography, Veterinary Information Network

Kittleson M

Small Animal cardiovascular

M

edicine, Veterinary Information Network. Chapter 3 – Signalment, History and Physical Examination