PPT-Electron Microscopy Chelsea Aitken

Peter Aspinall httpwwwzyvexlabscomEIPBNuGEIPBN20images05Ionjpg Intro to Electron Microscopy Similar to optical microscopy except with electrons rather than photons Used to image samples with a resolution of 10 Å. Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Lecture . 7: Confocal Microscopy. Optical Sectioning: adding the third dimension. Wide-field . Imaging. Point . Spread Function. Deconvolution. Chelsea Aitken. Peter Aspinall. http://www.zyvexlabs.com/EIPBNuG/EIPBN%20images/05Ion.jpg. Intro to Electron Microscopy. Similar to optical microscopy except with electrons rather than photons. Used to image samples with a resolution of 10 Å. Chelsea Aitken. Peter Aspinall. Advantages Over Light Microscopy. Resolution of light microscopy limited by Rayleigh Criterion. If two objects cannot be seen as distinct structures, then they may be considered coincident in space. Lecture 13:. Super-resolution microscopy: Part I. Lecture 13: . Fluorescent labeling, multi-. sprectral. imaging and FRET. Review of previous lecture. FRET. FLIM. Super resolution . microscopy. NSOM. Fire Protection Laboratory Methods Day. June 25, 2014. Paul M. Anderson. Graduate Research Assistant. University of Maryland. Department of Fire Protection Engineering. Transmission Electron Microscopy. Fire Protection Laboratory Methods Day. June 25, 2014. Paul M. Anderson. Graduate Research Assistant. University of Maryland. Department of Fire Protection Engineering. Transmission Electron Microscopy. Tutor: Peter Harris. Click on the buttons to move between slides. .. (Make sure you are in “Slide Show” mode). The aims of this course are:. ● . to explain how X-rays are generated in the electron microscope. phosphoglucosamine. mutase. Andrew Muenks. 1. , . Tommi. A. White. 1&2. , and Lesa J. Beamer. 1. Department of Biochemistry. 1. and Electron Microscopy Core. 2. , University of Missouri, Columbia, MO. Peter Harris. Transmission . electron . microscopy. ► What . is transmission electron microscopy?. . ► TEM in biological science . ► CfAM’s TEMs. ► TEM in physical . science . ► Recent developments in TEM. Outline. Introduction. Optical Microscopy. Types of Optical Microscopes. Confocal Microscopy. Laser Scanning Confocal Microscopy. Examples. Optical Microscopy. Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. (SEM) Electron Microscopy (SEM) and TEM Scanning electron microscopy is used for inspecting topographies of specimens at very high magnifications using a piece of equipment called the scanning electr Theoretically a microscope is an array of two lenses Need of M icroscopy Living organisms, cells and their organelles which are too small to be seen with the human eye, can only be seen with the aid O'Rourke LG, Pitulle C, Hegarty BC, Kraycirik S, Killary KA, Grosenstein P, et al. Bartonella quintana in Cynomolgus Monkey (Macaca fascicularis). Emerg Infect Dis. 2005;11(12):1931-1934. https://doi.org/10.3201/eid1112.030045. The aims of this course are:. ● . to introduce the principles of scanning electron microscopy. ● . to describe the components of the microscope and explain how they work. ● . to highlight some of the problems which can arise during imaging.