PPT-Transmission Electron Microscopy (TEM)

By Austin Avery Overview What is Transmission Electron Microscopy History of TEM Theory of TEM The instrumentation How is TEM useful ProsCons Summary Transmission Electron Microscopy TEM is an instrumental technique that uses a tiny focused beam of electrons. Chelsea Aitken. Peter Aspinall. http://www.zyvexlabs.com/EIPBNuG/EIPBN%20images/05Ion.jpg. Intro to Electron Microscopy. Similar to optical microscopy except with electrons rather than photons. Used to image samples with a resolution of 10 Å. Scanning Electron Microscopy (SEM). Uses. Sample Preparation. Instrument. Principles. Micrographs. Transmission Electron Microscopy (TEM). Uses. Sample Preparation. Instrument. Principles. Micrographs. phosphoglucosamine. mutase. Andrew Muenks. 1. , . Tommi. A. White. 1&2. , and Lesa J. Beamer. 1. Department of Biochemistry. 1. and Electron Microscopy Core. 2. , University of Missouri, Columbia, MO. -grown . InGaAs. quantum dot chains using transmission electron microscopy. Tyler Park. John Colton . Haeyeon. Yang. *. Jeff . Farrer. APS March Meeting 2013 Baltimore, MD. * South Dakota School of Mines. Introduction to transmission electron microscopy. Tutor: Peter Harris. The aims of this course are:. ● . to . describe the components of the transmission electron microscope (TEM). ● . to explain the origin of contrast in TEM images. Peter Harris. Transmission . electron . microscopy. ► What . is transmission electron microscopy?. . ► TEM in biological science . ► CfAM’s TEMs. ► TEM in physical . science . ► Recent developments in TEM. Outline. Introduction. Optical Microscopy. Types of Optical Microscopes. Confocal Microscopy. Laser Scanning Confocal Microscopy. Examples. Optical Microscopy. Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. . Electron microscopy (EM) . is an electron beam which is focused into a small probe across the surface of a specimen . . The first electromagnetic lens was developed in 1926 by . Hans Busch. .. . AIM : T THEORY: Microscope is the most commonly used piece of apparatus in the laboratory. It produces greatly enlarged images of minute objects. LIGHT MICROSCOPE A Light Microscope can be simple or (SEM) Electron Microscopy (SEM) and TEM Scanning electron microscopy is used for inspecting topographies of specimens at very high magnifications using a piece of equipment called the scanning electr Theoretically a microscope is an array of two lenses Need of M icroscopy Living organisms, cells and their organelles which are too small to be seen with the human eye, can only be seen with the aid The theory of image formation in electron microscope s is very similar to image formation in light microscope s. However, the practice and design are completely different : The most crucial Ng M, Lee J, Leong M, Ling A, Tan H, Ooi E. Topographic Changes in SARS Coronavirus–infected Cells during Late Stages of Infection. Emerg Infect Dis. 2004;10(11):1907-1914. https://doi.org/10.3201/eid1011.040195. ISAC_CS_08. Soot-in-Oil Diagnostics. Transmission Electron Microscopy (TEM) & Nanoparticle Tracking Analysis (NTA) Case Study. SOOT-IN-OIL CONTAMINATION. A build-up of . soot . in engine oil reduces performance..