PPT-Cryo-Electron Microscopy

James Conway University of Pittsburgh School of Medicine 1 Making images image formation contrast function detectors film amp scanning CCD DED energy filters phase plates 2 Making density maps AUTO3DEM. Chelsea Aitken. Peter Aspinall. Advantages Over Light Microscopy. Resolution of light microscopy limited by Rayleigh Criterion. If two objects cannot be seen as distinct structures, then they may be considered coincident in space. Fire Protection Laboratory Methods Day. June 25, 2014. Paul M. Anderson. Graduate Research Assistant. University of Maryland. Department of Fire Protection Engineering. Transmission Electron Microscopy. II. MENA3100,OBK, . 29.01.15. We don’t read all. You don’ have to read all. 1.3 Specimen Preparation. Read it. 1.4.1 Bright-Field and Dark-Field. Definetly. 1.4.2 Phase-Contrast. Cursori. 1.4.3 Polarized-Light. Tutor: Peter Harris. Click on the buttons to move between slides. .. (Make sure you are in “Slide Show” mode). The aims of this course are:. ● . to explain how X-rays are generated in the electron microscope. phosphoglucosamine. mutase. Andrew Muenks. 1. , . Tommi. A. White. 1&2. , and Lesa J. Beamer. 1. Department of Biochemistry. 1. and Electron Microscopy Core. 2. , University of Missouri, Columbia, MO. V.Parma, . CERN, TE-MSC. On behalf of the . SPL cryomodule development . team . (CERN, CEA-Saclay, CNRS-IPNO Orsay, ESS). Outline. SPL cryomodule goals and motivation. The collaboration effort. Main design features. Outline. Introduction. Optical Microscopy. Types of Optical Microscopes. Confocal Microscopy. Laser Scanning Confocal Microscopy. Examples. Optical Microscopy. Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. . Electron microscopy (EM) . is an electron beam which is focused into a small probe across the surface of a specimen . . The first electromagnetic lens was developed in 1926 by . Hans Busch. .. . AIM : T THEORY: Microscope is the most commonly used piece of apparatus in the laboratory. It produces greatly enlarged images of minute objects. LIGHT MICROSCOPE A Light Microscope can be simple or (SEM) Electron Microscopy (SEM) and TEM Scanning electron microscopy is used for inspecting topographies of specimens at very high magnifications using a piece of equipment called the scanning electr Theoretically a microscope is an array of two lenses Need of M icroscopy Living organisms, cells and their organelles which are too small to be seen with the human eye, can only be seen with the aid O'Rourke LG, Pitulle C, Hegarty BC, Kraycirik S, Killary KA, Grosenstein P, et al. Bartonella quintana in Cynomolgus Monkey (Macaca fascicularis). Emerg Infect Dis. 2005;11(12):1931-1934. https://doi.org/10.3201/eid1112.030045. A . fluorescence microscope.  is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to . study . organic . or inorganic substances. ELECTRON. . MICROSC. O. PY. . . . Electron. . micros. c. opy. . (EM). . is a technique. . u. s. ed. . for. . obtaining. . high re. s. olu. t. ion. . images. . of. . biological and non-biologi.