PPT-Cryo-Electron Microscopy

James Conway University of Pittsburgh School of Medicine 1 Making images image formation contrast function detectors film amp scanning CCD DED energy filters phase plates 2 Making density maps AUTO3DEM. Bob . Ashley AAS . SMFW. 7-13-2013. Reading List. Reading List . Negative Staining 1990 Hayat and Miller. Baker, T. S., and R. Henderson (2006). Electron cryomicroscopy. In "International Tables for Crystallography", Vol. F, ch. 19, pp. 451-463. Chelsea Aitken. Peter Aspinall. http://www.zyvexlabs.com/EIPBNuG/EIPBN%20images/05Ion.jpg. Intro to Electron Microscopy. Similar to optical microscopy except with electrons rather than photons. Used to image samples with a resolution of 10 Å. Fire Protection Laboratory Methods Day. June 25, 2014. Paul M. Anderson. Graduate Research Assistant. University of Maryland. Department of Fire Protection Engineering. Transmission Electron Microscopy. By Austin Avery. Overview. What is Transmission Electron Microscopy?. History of TEM. Theory of TEM. The instrumentation. How is TEM useful?. Pros/Cons. Summary. Transmission Electron Microscopy. TEM is an instrumental technique that uses a tiny focused beam of electrons.. Fire Protection Laboratory Methods Day. June 25, 2014. Paul M. Anderson. Graduate Research Assistant. University of Maryland. Department of Fire Protection Engineering. Transmission Electron Microscopy. phosphoglucosamine. mutase. Andrew Muenks. 1. , . Tommi. A. White. 1&2. , and Lesa J. Beamer. 1. Department of Biochemistry. 1. and Electron Microscopy Core. 2. , University of Missouri, Columbia, MO. Microscopy is the technical field using microscopes to view samples and objects that can not be seen without unaided eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron and scanning probe microscopy.. Replica of van 1670 Moody Use the information in this tutorial to supplement the visuals in lab and the Chapters 1 8 and 9 in your lab manual Replica of Culpepper tripod microscope built c 1725 by Co Peter Harris. Transmission . electron . microscopy. ► What . is transmission electron microscopy?. . ► TEM in biological science . ► CfAM’s TEMs. ► TEM in physical . science . ► Recent developments in TEM. . Electron microscopy (EM) . is an electron beam which is focused into a small probe across the surface of a specimen . . The first electromagnetic lens was developed in 1926 by . Hans Busch. .. . (SEM) Electron Microscopy (SEM) and TEM Scanning electron microscopy is used for inspecting topographies of specimens at very high magnifications using a piece of equipment called the scanning electr The theory of image formation in electron microscope s is very similar to image formation in light microscope s. However, the practice and design are completely different : The most crucial The aims of this course are:. ● . to introduce the principles of scanning electron microscopy. ● . to describe the components of the microscope and explain how they work. ● . to highlight some of the problems which can arise during imaging. ELECTRON. . MICROSC. O. PY. . . . Electron. . micros. c. opy. . (EM). . is a technique. . u. s. ed. . for. . obtaining. . high re. s. olu. t. ion. . images. . of. . biological and non-biologi.