PPT-Determination of serum Iron

Iron is an essential nutrient that is required for the production of healthy red blood cells RBCs It is a critical part of hemoglobin the protein in RBCs that binds oxygen in the lungs and releases it as blood circulates to other parts of the body A serum iron test measures how much iron is in your serum The serum iron test can reveal abnormally low or high blood iron levels . haemoglobinopathies. Dr Farrukh Shah. Consultant haematologist. Joint Red cell disorders unit. Whittington hospital and UCLH. Why?. . . . . Erythron. . Macrophages. Hepatocytes. & other. Protein . Nitrogen (NPN) . Concentration. 224 PHL. Lab#5 . Non-protein nitrogen (NPN). NPN includes . the nitrogen from all nitrogenous substances . other than proteins. ..  . The NPN could be measured as a . Total Protein . Concentration. 224 PHL. Lab#. 2. . 1) Simple Proteins:. . They yield only . α. -amino acids . on hydrolysis.. e.g. . albumin, globulin. .. 2) Conjugated Proteins:. . They are proteins combined with a non-protein substance called prosthetic group. . serum iron. , unsaturated iron binding capacity (. UIBC. ), and total iron binding capacity (. TIBC. ) . Objectives. To determine the normal level of serum iron. . To . determine the use of this test in diagnosis of anemia (iron deficiency). . IRON OVERLOAD. IRON. 10-15 mg/day in diet; 5-10% absorbed. Absorption increased in iron deficiency, pregnancy, erythroid hyperplasia, hypoxia. Heme iron absorbed best. Fe. 2 . much better than Fe. 3 . . . Abeer. . Anwer. Ahmed. There is no physiological mechanism for eliminating excess iron from the body, so iron absorption is normally regulated to avoid accumulation. . Iron overload (. haemosiderosis. Phosphatases. Activity . Phosphatases. . are enzymes which catalyze the splitting of phosphoric acid from mono-phosphate esters..  . They are . hydrolases. .. Organic phosphate esters + water alcohol + phosphate . DR. SANJANA BHAGWAT. MODERATOR : DR. A. . GANAPULE. Normal iron metabolism. IRON METABOLISM. Genetic forms of iron deficiency . anaemia. Mutations in genes encoding :. DMT-1. Glutaredoxin. 5. Transferrin. Welcome. Interpreting and Managing Blood Results – A Masterclass. Dr Karen McEwan, . Dr . Sayee Chirputkar & Dr Gill Burrows. Good Afternoon and welcome to this session. It is due to start at 12:30. . Head of Flow cytometry unit. Assistant professor & Consultant Hematologists. Hemoglobin??. α. β. β. α. Fe⁺⁺. Fe⁺⁺. Fe⁺⁺. Fe⁺⁺. Globin chain. Haem. Prophyrin. ring . Iron atom. bromocresol. green (BCG) dye binding method. .. One of the most important serum proteins produced in the liver and It is protein that is water soluble. *Functions:. -Nutrition. -Regulation of osmotic pressure. COLLEGE OF MEDICINE. DEPARTMENT OF PATHOLOGY AND FORENSIC. Medicine. Dr. . Ala’a. . A.Razak. . Abood. Iraqi Board of . Haematopathology. Objectives. :. 1- Define microcytic anemia and give differential diagnosis of microcytosis.. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are enzymes found mainly in the liver, but also found in red blood cells, heart cells, muscle tissue and other organs, such as the pancreas and kidneys. AST and ALT formerly are called serum glutamic . 224 PHL. Lab#. 1. . Introduction . Instrument . used in Colorimetric . method: . Spectrophotometer. Blood. Urine. Cerebrospinal fluid (CSF). Saliva. Types of Specimens used in the laboratory experiments:.