PPT-Enzymes Lab # 9 224 PHL Determination of Serum

Phosphatases Activity Phosphatases are enzymes which catalyze the splitting of phosphoric acid from monophosphate esters   They are hydrolases Organic phosphate esters water alcohol phosphate . and CNS . stimulants . Presented by. Mohammed . A. . Alyami. . Head Teaching Assistant. Department of Pharmacology & Toxicology. College of Pharmacy. KSU. E-mail : . maalyami@ksu.edu.sa. . All Courses of Pharmacology. Protein . Nitrogen (NPN) . Concentration. 224 PHL. Lab#5 . Non-protein nitrogen (NPN). NPN includes . the nitrogen from all nitrogenous substances . other than proteins. ..  . The NPN could be measured as a . Warm up. Grab warm up and complete. We will be putting this into your . journal. When you are done with your warm up look over your Vocab 1-1, you have a . quiz today. Biomolecule review. Cellulose, a structural component of the cell wall of plants and some forms of algae, is mainly used to produce paper and cardboard products. Cellulose would be an example of which type of biomolecule. Apoenzymes. , . Holoenzyme. , . Abzymes. , . Synzyme. , . Ribozyme. , . Extremozyme. , Historical Development in . Enzymology. , . Characteristics of enzymes, Enzyme Vs Chemical Catalysts. , Enzyme mechanism & Activation Energy, Enzyme Specificity, Fischer and . Total Protein . Concentration. 224 PHL. Lab#. 2. . 1) Simple Proteins:. . They yield only . α. -amino acids . on hydrolysis.. e.g. . albumin, globulin. .. 2) Conjugated Proteins:. . They are proteins combined with a non-protein substance called prosthetic group. . INDEX. Intoduction to culture media.. Components of serum.. Advantages and disadvantages of serum media.. Advantages and disadvantages of serum free media.. CULTURE MEDIA = defined as growth media suitable for the in- vitro cell culturing of human or animal cells and the composition of components are known.. . Name: . . Date: . . Block: . Title. : . Include the following terms: . Dissolving or breakdown of . gelatin. ( this is our DV). Pineapple (Fresh, Canned, Cooked, Juice). 1. What are enzymes?. Each enzyme has only one specific reaction it can perform. . 2. How many different reactions can a single enzyme perform?. Yes, the same enzyme molecule can be used over and over. Part 1. . Chapter 3. Learning outcomes. You should be able to:. explain how enzymes work. describe and explain the factors that affect. enzyme activity. use V. max. and K. m. to compare the affinity of different enzymes for their substrates. By. Zohreh. . Rahimi. Professor of Clinical Biochemistry. 1. TESTS OF LIVER INJURY. PLASMA ENZYME LEVELS. As metabolically complex cells, hepatocytes contain high levels of a number . of . enzymes. With liver injury, these enzymes may leak into plasma and can . Enzyme & Substrate. An enzyme is a globular protein which acts as a biological catalyst by speeding up the rate of chemical reactions. . Enzymes are . NOT. changed or consumed by the reactions they catalyze and thus can be reused. . Restriction enzymes. Polymerase enzyme. Strand-displacing polymerases. Helicase enzymes. Discovery and Obtainability:. Most enzymes are proteins discovered in cells.. But . DNAzymes. were . descovered. Measurements of enzymes. in serum. within a tissue. enzymes as markers of disease,. Injury to tissue releases cellular substances that can be used as plasma markers of tissue damage.. enzyme release is highly specific for cell death in some settings.. Tuberculosis Laboratory Aggregate Report ii Suggested Citation: CDC. Fourth Edition of the Tuberculosis Laboratory Aggregate Report, Atlanta, Georgia: U.S. Department of Health and Human Services, C