PPT-Enzymes Lab # 9 224 PHL Determination of Serum

Phosphatases Activity Phosphatases are enzymes which catalyze the splitting of phosphoric acid from monophosphate esters   They are hydrolases Organic phosphate esters water alcohol phosphate . (Diagnostic & Therapeutic ) . importance of . Enzymes and isoenzymes. Objectives. List the clinically important enzymes and . isoenzymes. .. State which of the enzymes and . isoenzymes. are found in which tissues. Protein . Nitrogen (NPN) . Concentration. 224 PHL. Lab#5 . Non-protein nitrogen (NPN). NPN includes . the nitrogen from all nitrogenous substances . other than proteins. ..  . The NPN could be measured as a . Total Protein . Concentration. 224 PHL. Lab#. 2. . 1) Simple Proteins:. . They yield only . α. -amino acids . on hydrolysis.. e.g. . albumin, globulin. .. 2) Conjugated Proteins:. . They are proteins combined with a non-protein substance called prosthetic group. . By. Zohreh. . Rahimi. Professor of Clinical Biochemistry. 1. TESTS OF LIVER INJURY. PLASMA ENZYME LEVELS. As metabolically complex cells, hepatocytes contain high levels of a number . of . enzymes. With liver injury, these enzymes may leak into plasma and can . By. Zohreh. . Rahimi. Professor of Clinical Biochemistry. 1. TESTS OF LIVER INJURY. PLASMA ENZYME LEVELS. As metabolically complex cells, hepatocytes contain high levels of a number . of . enzymes. With liver injury, these enzymes may leak into plasma and can . May Alrashed. PhD. General properties. Enzymes are protein catalyst that increase the velocity of a chemical reaction.. Enzymes are not consumed during the reaction they catalyzed.. With the exception of catalytic RNA molecules, or ribozymes, enzymes are proteins.. Measurements of enzymes. in serum. within a tissue. enzymes as markers of disease,. Injury to tissue releases cellular substances that can be used as plasma markers of tissue damage.. enzyme release is highly specific for cell death in some settings.. Professor of the Chemistry Department of . DonNMU. . BORZENKO BERTA GEORGIYEVNA. Co-authors: associate . prof. . Elena . Bakurova. , assistant . Kseniia. . Mironova. . . Donetsk National Medical University named after M. Gorky. (1992) 35:991-995 in plasma lysosomal enzymes in Type 1 (insulin-dependent) diabetes mellitus: relationship to diabetic complications and glycaemic control J. Waters 1 , M. D. Flynn 2, R. J. M. Corra Enzymes are catalysts that increase the rate of physiologic reactions. Each and every reaction in our body catalyzed by enzyme.. ☼ . In general, most enzymes are present in cells at much higher concentrations than in plasma.. bromocresol. green (BCG) dye binding method. .. One of the most important serum proteins produced in the liver and It is protein that is water soluble. *Functions:. -Nutrition. -Regulation of osmotic pressure. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are enzymes found mainly in the liver, but also found in red blood cells, heart cells, muscle tissue and other organs, such as the pancreas and kidneys. AST and ALT formerly are called serum glutamic . Iron is an essential nutrient that, is required for the production of healthy red blood cells (RBCs). It is a critical part of hemoglobin, the protein in RBCs that binds oxygen in the lungs and releases it as blood circulates to other parts of the body. A serum iron test measures how much iron is in your serum. The serum iron test can reveal abnormally low or high blood iron levels. . 224 PHL. Lab#. 1. . Introduction . Instrument . used in Colorimetric . method: . Spectrophotometer. Blood. Urine. Cerebrospinal fluid (CSF). Saliva. Types of Specimens used in the laboratory experiments:.