Department of Pathology and Laboratory Medicine Molecular Team Top Dr Paul Rothberg Bonnie Nuccie Middle Sharon Todd Nufatt Paige Bottom Suni Yanyun Bill John What is Molecular Diagnostics ID: 780156
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Molecular Diagnostics LaboratoryDepartment of Pathology and Laboratory Medicine
Molecular Team
Top: Dr. Paul Rothberg, Bonnie
Nuccie
Middle: Sharon, Todd,
Nufatt
, Paige
Bottom:
Suni
,
Yanyun
, Bill, John
What is Molecular Diagnostics?
Molecular diagnostic testing is used to diagnose and
monitor oncological and genetic diseases. It helps determine
risk and prognosis.
By
analyzing the individual's genetic code and how their cells express their genes as proteins, molecular diagnostics allows for personalized medicine .
How Is DNA Analyzed?
DNA is first extracted from the blood, bone marrow, or tissue samples. The polymerase
chain reaction (PCR
) is the technique used for the amplification of DNA to make millions of replicates of a target DNA sequence. Specific mutations which can be associated with certain diseases are detected once
the DNA is
amplified.
H
ow Do We Help Our Patients?
Bone Marrow Transplants: Molecular diagnostics plays a large role in stem cell transplants by determining whose cells are populating the bone marrow post transplant (donor or recipient?) This difference helps the treatment team determine the next steps for a successful transplant. Oncology: Molecular testing can diagnose and help guide therapy for patients with all varieties of cancer (lung, colon, brain, skin, blood, etc.)Genetics: There are certain genetic mutations that are risk factors for bleeding and iron overload disorders. Molecular diagnostics can help determine if the patient has the mutation and the likelihood the patient will develop the disease.
DNA sequencing analysis
Post PCR gel electrophoresis
Real time PCR of the
KRAS
gene