PPT-Supplemental Table 1 Primers used in Real Time RT-PCR analysis

Gene 　 Primer Sequence TERT Sense ACGGTGTGCACCAACATCTACAA Antisense TCAGAGATGACGCGCAGGA CDK4 Sense GCCTGGCCAGAATCTACAGCTAC Antisense  GGTCGGCTTCAGAGTTTCCAC CDH1 Sense TACACTGCCCAGGAGCCAGA. How are PCR Arrays Utilized The RT57522 Profiler PCR Arrays have been increasingly used in research on cancer immunology stem cells toxicology biomarker discovery and validation and phenotypic analysis of cells and transgenic animals Why PCR Arrays . Hox. gene . DthoxC. insertion into prokaryote . E.coli. . – by . UNIamCloning. G.Tigrina. & the . Hox. genes. G. . tigrina. – (also known as . Dugesia. . tigrina. ) – are free-living flatworms found in still fresh water. Commonly used in biology teaching labs, these planarians show a profound capability to regenerate . PCR provides a forensics tool for identifying colonies. Three strains look alike!. How can you identify the strains?. Geneticists like to verify their strains’ genotypes before experiments. Photo by Yellowstone NPS. of . Genomic . DNA Fragments. OrR. Amplification. To get particular DNA in large amount. Fragment size shouldn’t be too long. The nucleotide sequence at each end is known.. How to get known sequence from both end of particular genome fragment?. Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . "molecular photocopying" . It’s fast, inexpensive and simple . Polymerase Chain Reaction. Amplifying DNA . in Vitro. : The Polymerase Chain Reaction (PCR). The . polymerase chain reaction, PCR. , can produce many copies of a specific target segment of DNA. Real-Time PCR is a specialized technique that allows a PCR reaction to be visualized “in real time” as the reaction progresses.. This enables researchers to quantify the amount of DNA in the sample at the start of the reaction!. reproduce modified versions of Figures 4-3, 11-12, 12-3 (a), 12-6, 12-7 (c), 12-7 (d), 14-20, 14-24, 14-29 and 17-10 (a) from the book published by them in 1996 with the titleIntroduction to Genetic A Plaut RD, Staab AB, Munson MA, Gebhardt JS, Klimko CP, Quirk AV, et al. Avirulent Bacillus anthracis Strain with Molecular Assay Targets as Surrogate for Irradiation-Inactivated Virulent Spores. Emerg Infect Dis. 2018;24(4):691-699. https://doi.org/10.3201/eid2404.171646. PCR. GENE TECHNIQUES . . Dr. Nadal Abdulameer Ali. . The primers are the key to the success or failure of a PCR experiment. If the primers. are designed correctly the experiment results in amplification of a single DNA frag-. Fryer JF, Kapoor A, Minor PD, Delwart E, Baylis SA. Novel Parvovirus and Related Variant in Human Plasma. Emerg Infect Dis. 2006;12(1):151-154. https://doi.org/10.3201/eid1201.050916. Three strains look alike!. How can you identify the strains?. Geneticists like to verify their strains’ genotypes before experiments. Photo by Yellowstone NPS. Discovery of the polymerase chain reaction expanded the reach of molecular biologists . A.. B.. Supplemental Figure 3. Control. α-synuclein. Vehicle. Nicotine. A.. B.. C.. D.. No RNAi. SV2L1 KD. SV2L2 KD. Target . gene. . name. Forward primer. Reverse primer . RP11-146N23.4. TGTTTTGTCTCCGGTGTTCCA. ATAAGGCCAGGTGCGGTG. RP11-513M16.8. TATCTGCGCCTTAACCAGACC. AATGCAAGCTCTTTGTTGGCA. RP11-77K12.9. CCATGAAAGTCGGCCCAAGA.