Early Development 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E Plasma membrane Outer acrosomal membrane Acrosome Inner acrosomal membrane Nucleus Proximal centriole Rest of the distal ID: 811032
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Slide1
Sperm Cell StructureFertilizationEarly Development
Slide2Slide312
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89101112ABCDEPlasma membraneOuter acrosomal membraneAcrosomeInner acrosomal membraneNucleusProximal centrioleRest of the distal centrioleThick outer longitudinal fibersMitochondrionAxonemeAnulusRing fibersHeadNeckMid piecePrincipal piece Endpiece
Slide4Slide5Fertilization
Slide6Sperm CapacitationFreshly ejaculated sperm are unable or poorly able to fertilize. They must first undergo a series of changes known collectively as capacitation
.
Capacitation
is associated with removal of
proteins
, reorganization of plasma membrane lipids and proteins.
Slide7Sperm-Zona Pellucida BindingBinding of sperm to the zona
pellucida
is a receptor-
ligand
interaction
The carbohydrate groups on the zona pellucida glycoproteins function as sperm receptors. The sperm molecule that binds this receptor is not known with certainty, and indeed, there may be several proteins that can serve this function.
Slide8The Acrosome ReactionThe sperm then faces the daunting task of penetrating the zona
pellucida
to get to the
oocyte
.
The
acrosome - a huge modified lysosome that is packed with zona-digesting enzymesThe acrosome reaction provides the sperm with an enzymatic drill to get throught the zona pellucida. Leakage of acrosomal enzymes from the sperm's head.As the acrosome reaction progresses and the sperm passes through the zona pellucida, more and more of the plasma membrane and acrosomal contents are lost. Some sperm that lose their acrosomes and are not effective
Slide9Penetration of the Zona PellucidaThe force from the sperm's flagellating tail
+
acrosomal
enzymes, allow the sperm to create a tract through the
zona
pellucida. These two factors allow the sperm to traverse the zona pellucida. Sperm motility is important to zona penetration, allowing the sperm to basically cut its way through the zona
Slide10Sperm-Oocyte BindingOnce a sperm penetrates the zona pellucida, it binds to and fuses with the plasma membrane of the
oocyte
.
The molecular nature of sperm-
oocyte
binding is not completely resolved.
A leading candidate in some species is a dimeric sperm glycoprotein called fertilin, which binds to a protein in the oocyte plasma membrane and may also induce fusion.
Slide11The Zona ReactionThe zona reaction refers to an alteration in the structure of the zona
pellucida
catalyzed by proteases from cortical granules.
blocks
polyspermy
in most mammals.
The zona pellucida hardens. Runner-up sperm that have not finished traversing the zona pellucida by the time the hardening occurs are stopped in their tracks.Sperm receptors in the zona pellucida are destroyed. Therefore, any sperm that have not yet bound to the zona pellucida will no longer be able to bind, let alone fertilize the egg.
Slide12Cleavage and Blastocyst Formation
Slide13Slide14The one cell embryo undergoes a series of cleavage divisions, progressing through 2-cell, 4-cell, 8-cell and 16 cell stages. A four cell embryo is shown here. The cells in cleavage stage embryos are known as blastomeres.
Slide15Early on, cleavage divisions occur quite synchronously. In other words, both blastomeres in a two-cell undergo mitosis and cytokinesis almost simultaneously.
Slide16Soon after development of the 8-cell or 16-cell embryo the formation becomes a mass of cells called a morula. this embryo shown here probably has between 20 and 30 cells.
It is difficult to count the cells in a
morula
;
Slide17Formation of an accumulation of fluid inside the embryo, signals formation of the blastocyst.
Slide18Implantation
Slide19