Data Analysis Mitotic Index The Mitotic Index M represents the proportionfrequency of mitotic cells in your cell population M n m N n m the number of mitotic cells in the sample ID: 920011
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Slide1
Mechanisms of Mitosis
Mitotic Indices
Slide2Data Analysis: Mitotic Index
The
Mitotic Index (M)
represents the proportion/frequency of mitotic cells in your cell population.
M = n
m
/N
n
m
= the number of mitotic cells in the sample
(i.e., those not in interphase)
N
= the total number of cells counted in the sample.
Slide3Data Analysis: Mitotic Index
Why might it be useful to compare
mitotic indices
between treatment and control groups?
Slide4Data Analysis: Mitotic Phase Index
A
Mitotic Phase Index (M
P
)
can be calculated for each mitotic phase you encounter.
For example, a prophase index would be:
M
pro
=
n
pro
/n
m
n
pro
= the number of mitotic cells in prophase in the sample
n
m
= the total number of mitotic cells in the sample
Slide5Data Analysis: Mitotic Index
Why might it be useful to compare
mitotic phase indices
between treatment and control groups?
Slide6Data Analysis: Mitotic Index
Do you need to compare
all phases
between treatment and control?
Slide7Data Analysis: Mitotic Index
Which phases
would be most useful to compare, given your hypothesis?
Slide8Data Analysis: Mitotic Index
In today’s lab, your team will calculate indices for
12 control
onion root tips
12 treatment
onion root tips
…from pooled data collected by all teams in your lab section.
Slide9Data Analysis: Mitotic Index
Use the worksheets linked in your online lab manual to record and tabulate
Mitotic indices
Mitotic phase indices
(as appropriate)
Slide10Tabulate Mitotic Indices
Sample #
(from one onion)
Treatment
(mitotic
index )
Control
(mitotic index
)
1
0.20
0.55
2
0.25
0.60
3
0.45
0.65
4
0.35
0.80
5
0.15
0.35
6
0.10
0.75
7
0.55
0.70
8
0.40
0.85
9
0.30
0.90
10
0.45
0.50
Slide11Data Analysis:
Mitotic Phase Index
Decide which
Mitotic Phase Indices (
M
phase
)
to
use fo
r control and treatment comparison
…
Prophase
Metaphase
Anaphase
Telophase
Interphase
Slide12Data Analysis:
Mitotic Phase Index
Use the Worksheets linked in your online lab manual to record and tabulate indices.
Slide13Example:
PROphase
Mitotic Phase Index
Sample #
(from one onion)
Treatment
(M
P
for cells in
prophase
)
Control
(M
P
for cells in
prophase
)
1
0.20
0.55
2
0.25
0.60
3
0.45
0.65
4
0.35
0.80
5
0.15
0.35
6
0.10
0.75
7
0.55
0.70
8
0.40
0.85
9
0.30
0.90
10
0.45
0.50
Slide14Your Indices are Your Data
Mitotic Indices
and
Mitotic Phase Indices
of
treatment samples
control samples
…
are the data you will statistically analyze with a
Mann-Whitney U test
.
Slide15Your Indices are Your Data
Make sure each team member has access to a copy of his/her/their team’s index calculations before leaving lab.
Slide16Questions?