PPT-Cloning and expression of

Human insulin gene Diabetes and the role of insulin Diabetes mellitus Characterized by the excretion of large amount of glucose in urine Resulted from the bodys inability to make sufficient insulin . (Part 1). Created by: Haley . Vrazel. Objectives. Define cloning.. Identify the history of cloning. . Analyze the different types of cloning.. What is Cloning?. Animal cloning is an assisted reproductive . In biotechnology, cloning refers to the different processes used for . duplicating . biological material . (ex. DNA fragments, cells or organisms). . . The 3 Types of Cloning Technologies:. 1. Recombinant DNA Technology/DNA Cloning. Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . Dr. E. What is a plasmid?. Small double stranded circular DNA. Naturally found in bacteria. It is completely separate form bacterial chromosomal DNA. It independently self replicates . May give bacteria a . Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . A number of different processes used to produce genetically identical copies of biological material. Researchers have cloned genes, cells, . tissues, and entire organisms. Do clones ever occur naturally?. describe the production of natural clones in plants using the example of vegetative propagation in elm trees; . describe the production of artificial clones of plants from tissue culture;. discuss the advantages and disadvantages of plant cloning in agriculture (HSW6a, 6b, 7c); . Lecture #9. Lehninger. . Principles of Biochemistry. Chapter 9. DNA-based information technologies. Genomics & Proteomics. Genome. : . A genome is the complete set of genetic information in an organism.. Chapter 5: Investigating DNA. Major Topics. 5.1 DNA Isolation,  Sequencing, and Synthesis. 5.2 Bioinformatics. 5.3 Cloning and Recombinant Expression. 5.4 Microarrays. 5.5 In Situ Hybridization. Foundations of Biochemistry. Mammalian Cells. Gang Zhang, Ph. D. Research Technician II. Centre for Research in Neurodegenerative Diseases. Department of Medicine. University of Toronto, Canada. This talk based on the following publications:. has four essential parts: . a method for . generating the DNA fragment for cloning; . A reaction . that inserts that fragment into the . chosen cloning vector. . a . means for introducing . that recombinant . DEFINITION. HISTORY. STEPS . PROCEDURE. TYPES . EXAMPLES . ADVANTAGES AND DISADVANTAGES. WORD ORIGINATION. The term clone, invented by J. B. S. Haldane, is derived from the Ancient Greek word "twig", referring to the process whereby a new plant can be created from a twig.. A vector is a DNA molecule that has the abil­ity to replicate autonomously in an appropri­ate host cell and into which the gene of inter­est (a foreign genetic sequence) is integrated. When we insert a foreign genetic sequence into the vector the aim is either to obtain numer­ous copies of the gene of interest or to obtain the product of that.. Single celled, and easy to grow like bacteria. Yeast strains are genetically well characterized. Detailed genetic maps are. available for . Saccharomyces. . cerevisiae. and . Schizosaccharomyces. .