PPT-Structure of the antibody fragment (Fab 1A12) interacting with its target protein antigen

Research Details A vaccinederived human antibody fragment Fab 1A12 was crystallized alone and in complex with its target antigen factor H binding protein fHbp In a previous study Fab 1A12 crossreacted with fHbp variants that have different aminoacid sequences antibodies are usually specific to one fHbp variant group. . Using the characteristic of high affinity and specificity of antibody, we can detect or quantitative the antigen. Keep in mind that the antibody is protein, can also be recognized as an antigen. The major principle to determine the antigen-antibody interaction is to separate the bound form of antigen-antibody complex from the free form of either antigen or antibody. . Group . 3. :. M. . Yusron. . Hasani. . (. 105090100111037. ). A. Muammar . Kadafi. . (115090101111012. ). Nira. . Meirit. a. W.. . . (. 115090100111017. ). Putri. . Indisari. . (. 1150901. Sequence:. Edman degradation. Mass spectrometry. Secondary structure:. Circular Dichroism. FTIR. Tertiary, quaternary structure:. NMR. X-ray crystallography. Protein sequencing approaches depend on what is known and what is the goal. Sanjeev. Kumar. Cadila. . Healthcare Ltd.. September 1, 2012. Beyond Antibodies. Chapter 1. Conventional Antibodies. Antibodies. Structure. Structure-Function Correlation. Evolution. Terminology. Structure of an Antibody Molecule. reactions. Let’s start. The immune system is a system of biological structures and processes within an organism that protects against disease.. . To function properly, an immune system must detect a wide variety of agents, from viruses to parasitic worms, and distinguish them from the organism’s own healthy tissue. . Sequence:. Edman degradation. Mass spectrometry. Secondary structure:. Circular Dichroism. FTIR. Tertiary, quaternary structure:. NMR. X-ray crystallography. Protein sequencing approaches depend on what is known and what is the goal. Let’s start. The immune system is a system of biological structures and processes within an organism that protects against disease.. . To function properly, an immune system must detect a wide variety of agents, from viruses to parasitic worms, and distinguish them from the organism’s own healthy tissue. . Associate Professor,. Department of Chemistry,. Harish Chandra PG College, Varanasi. IMMUNOASSAY. The immunoassay technique are important for the analysis of:. . Harmones. . Drugs. Dr. Suzan Y.. Antigen. :. It s a substance that may be specifically bound by an antibody (. Ab. ) molecule or T cell receptors (TCR). When this binding lead to the activation of B cells or T cells ,this substance is known as . ELISA. Immunofluorescence. Flow cytometry. Western blotting. ELISA. We use an antibody that’s bound covalently to an enzyme to bind:. -antigen (direct ELISA). -antibody that is bound to antigen (indirect ELISA). Western blotting, also known as . immunoblotting. or protein blotting, is a core technique in cell and molecular biology. In most basic terms, it is used to detect the presence of a specific protein in a complex mixture extracted from cells. . What are the structural characteristics of antibodies?. Reading: Immunobiology textbook Chapter 4: “Antigen recognition by B cell and T cell receptors”. A. Immunoglobulin structure. 1. subunit structure. Detection By. Precipitation Methods. Dr.T.V.Rao MD. 11/11/2014. Dr.T.V.Rao MD. 1. Beginning of Serology. Serology as a science began in 1901. Austrian American immunologist . Karl Landsteiner. (1868-1943) identified groups of red blood cells as A, B, and O. From that discovery came the recognition that cells of all types, including blood cells, cells of the body, and microorganisms carry proteins and other molecules on their surface that are recognized by cells of the . UNIT-2. : . . Techniques in Clinical Microbiology. . Complement fixation . reaction. . . - . Page no. 112-113. . Immunofluorescence. . - . Page no. 118-119. . Enzyme linked immunosorbent assay (ELISA.