PPT-Structure of the antibody fragment (Fab 1A12) interacting with its target protein antigen

Research Details A vaccinederived human antibody fragment Fab 1A12 was crystallized alone and in complex with its target antigen factor H binding protein fHbp In a previous study Fab 1A12 crossreacted with fHbp variants that have different aminoacid sequences antibodies are usually specific to one fHbp variant group. . Using the characteristic of high affinity and specificity of antibody, we can detect or quantitative the antigen. Keep in mind that the antibody is protein, can also be recognized as an antigen. The major principle to determine the antigen-antibody interaction is to separate the bound form of antigen-antibody complex from the free form of either antigen or antibody. . (such as bacteria,. fungi, and viruses). INNATE IMMUNITY. (all animals). • Rapid response. Recognition of traits shared. by broad ranges of. pathogens, using a small. set of receptors. •. Recognition of traits . Sequence:. Edman degradation. Mass spectrometry. Secondary structure:. Circular Dichroism. FTIR. Tertiary, quaternary structure:. NMR. X-ray crystallography. Protein sequencing approaches depend on what is known and what is the goal. 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 0.88 0.9 0.92 0.94 0.96 0.98 1 FAB-MAP FAB-MAP 3D with Delaunay Tessellation FAB-MAP 3D with Complete Graph Evaluation 0 50 100 150 200 250 1000 5000 10000 South Korea : Current Status and Potential. About Me. Hunmin. . Koh. KAIST. . 2004-2009. (Korea Advanced Institute of Science and Technology). - B.E in Mechanical Engineering. SNU. . . 2009-. (. V.. 0.0). Institute for . HyperNetwork. . Society. Oita Prefecture/City. P. opulation: . 1.2M / 0.4M. Institute for . HyperNetwork. Society. Origin: 1985 – COARA Grass-root citizens making “Virtual Community”. . Lab. 2. Immunoassay. An immunoassay is a test that uses antibody and antigen complexes as a . mean . of generating a measurable . result. Immuno. . refers to an immune response that causes the body to generate antibodies, . reactions. Let’s start. The immune system is a system of biological structures and processes within an organism that protects against disease.. . To function properly, an immune system must detect a wide variety of agents, from viruses to parasitic worms, and distinguish them from the organism’s own healthy tissue. . Pieter van der Hijden (pvdh@sofos.nl). 1. Fab7, Lima, Peru, 2011. Fab Lab Suriname. Waag NL with local partner. Pieter van der Hijden (pvdh@sofos.nl). 2. Fab7, Lima, Peru, 2011. Fab Lab Suriname. Help needed. By the end of this session student should be able to know. . Definition of antigen. . Factors influencing immunogenicity. Biological classes of antigens. . ANTIGEN. Defined as any substance that satisfies two distinct immunologic . Associate Professor,. Department of Chemistry,. Harish Chandra PG College, Varanasi. IMMUNOASSAY. The immunoassay technique are important for the analysis of:. . Harmones. . Drugs. Dr. Suzan Y.. Antigen. :. It s a substance that may be specifically bound by an antibody (. Ab. ) molecule or T cell receptors (TCR). When this binding lead to the activation of B cells or T cells ,this substance is known as . Dr. . Amany. . Ballow. . Consultant Immunology . Assistant professor KKUH. General Lab Director MDLAB. PhD, Diploma BSHI,MSC.. Objectives . To describe B-cells as the mediators of humoral immunity, (antibody-mediated immunity). UNIT-2. : . . Techniques in Clinical Microbiology. . Complement fixation . reaction. . . - . Page no. 112-113. . Immunofluorescence. . - . Page no. 118-119. . Enzyme linked immunosorbent assay (ELISA.