PPT-Antigen-antibody

reactions Lets start The immune system is a system of biological structures and processes within an organism that protects against disease To function properly an immune system must detect a wide variety of agents from viruses to parasitic worms and distinguish them from the organisms own healthy tissue . . Lab. 2. Immunoassay. An immunoassay is a test that uses antibody and antigen complexes as a . mean . of generating a measurable . result. Immuno. . refers to an immune response that causes the body to generate antibodies, . BCH 462 [practical] . Lab#7. Objective:. -To get familiar with radial immunodiffusion.. -To determine the concentration of unknown antigen.. . -Precipitation . reactions are based on the interaction of antibodies and antigens. They are based on two soluble reactants that come together to make one insoluble product, the precipitate (. BCH 462 [practical] . Lab#7. Objective:. -To get familiar with radial immunodiffusion.. -To determine the concentration of unknown antigen.. . Antigen [Ag] molecules each have a set of antigenic determinants or epitopes..  . Enzyme-Linked . ImmunoSorbant. Assay (ELISA). ELISA. ELISA - an acronym for . E. nzyme-. L. inked . I. mmuno. S. orbent. . A. ssay.. The ELISA assay is a widely used biochemical assay to detect in a sample the presence of and quantity of proteins, such as hormones and antibodies and bacteria or viruses.. adio. i. mmuno. a. ssay. . Remember. . Immunoassay reactions may be competitive or non-competitive. Competitive. . . labeled known and patient unknown are added to reaction and “compete” for the target.. and . Immunology. 1. 36. Copyright © McGraw-Hill Global Education Holdings, LLC. Permission required for reproduction or display.. The Clinical . Microbiology Laboratory. Clinical . microbiologist. major function is to isolate and identify microbes from clinical specimens rapidly. Agglutination. The interaction between an antibody and a particulate antigen results in visible clumping called agglutination. Particulate antigen include: . Bacteria, . W. hite blood cells, . R. ed blood cells, . . Response occurs within days of the infection. . Highly specific. . Highly diverse. . Memory component. . Major cell types involved: T cells, B cells and antigen presenting cells. Antigens and Antibodies (CH4). Let’s start. The immune system is a system of biological structures and processes within an organism that protects against disease.. . To function properly, an immune system must detect a wide variety of agents, from viruses to parasitic worms, and distinguish them from the organism’s own healthy tissue. . By the end of this session student should be able to know. . Definition of antigen. . Factors influencing immunogenicity. Biological classes of antigens. . ANTIGEN. Defined as any substance that satisfies two distinct immunologic . Associate Professor,. Department of Chemistry,. Harish Chandra PG College, Varanasi. IMMUNOASSAY. The immunoassay technique are important for the analysis of:. . Harmones. . Drugs. ELISA. Immunofluorescence. Flow cytometry. Western blotting. ELISA. We use an antibody that’s bound covalently to an enzyme to bind:. -antigen (direct ELISA). -antibody that is bound to antigen (indirect ELISA). Iraqi Center for Cancer and Medical Genetics Researches. Application of ELISA. Presence of antigen or the presence of antibody in a sample can be evaluated.. Determination of serum antibody concentrations in a virus test.. Detection By. Precipitation Methods. Dr.T.V.Rao MD. 11/11/2014. Dr.T.V.Rao MD. 1. Beginning of Serology. Serology as a science began in 1901. Austrian American immunologist . Karl Landsteiner. (1868-1943) identified groups of red blood cells as A, B, and O. From that discovery came the recognition that cells of all types, including blood cells, cells of the body, and microorganisms carry proteins and other molecules on their surface that are recognized by cells of the .