PPT-Antigen and Antibody Reactions

Detection By Precipitation Methods DrTVRao MD 11112014 DrTVRao MD 1 Beginning of Serology Serology as a science began in 1901 Austrian American immunologist Karl Landsteiner 18681943 identified groups of red blood cells as A B and O From that discovery came the recognition that cells of all types including blood cells cells of the body and microorganisms carry proteins and other molecules on their surface that are recognized by cells of the . . Lab. 2. Immunoassay. An immunoassay is a test that uses antibody and antigen complexes as a . mean . of generating a measurable . result. Immuno. . refers to an immune response that causes the body to generate antibodies, . . BCH462 [practical]. Objective:. - To learn the technique of . immunoelectrophoresis. .. -Precipitation . reactions are based on the interaction of antibodies and antigens. They are based on two soluble reactants that come together to make one insoluble product, the precipitate (. Agglutination. The interaction between an antibody and a particulate antigen results in visible clumping called agglutination. Particulate antigen include: . Bacteria, . W. hite blood cells, . R. ed blood cells, . Agglutination. The interaction between antibody and a particulate antigen results in visible clumping called . agglutination. Particulate antigen include: . bacteria, . white blood cells, . red blood cells, . . Response occurs within days of the infection. . Highly specific. . Highly diverse. . Memory component. . Major cell types involved: T cells, B cells and antigen presenting cells. Antigens and Antibodies (CH4). Immunoassay. An immunoassay is a test that uses antibody and antigen complexes as a mean of generating a measurable result. Immuno. refers to an immune response that causes the body to generate antibodies, . Let’s start. The immune system is a system of biological structures and processes within an organism that protects against disease.. . To function properly, an immune system must detect a wide variety of agents, from viruses to parasitic worms, and distinguish them from the organism’s own healthy tissue. . how to avoid them. What is the problem?. We have a reproducibility crisis in science. The consequence is a enormous waste in materials, time and money, costing an estimated . US$350 million. annually in the US alone!. Dr. Suzan Y.. Antigen. :. It s a substance that may be specifically bound by an antibody (. Ab. ) molecule or T cell receptors (TCR). When this binding lead to the activation of B cells or T cells ,this substance is known as . UNIT- . IV. ANTIGEN-ANTIBODY . REACTION . II. (Agglutination Reaction ). Prof. . D.A.Chouhan. . M.Sc., NET JRF (CSIR), NET JRF ( UGC), GATE, DMLT . Tagged antibody test: . In previous ppts we discuss about untagged serological reactions. These reactions has following imitations.. ELISA. Immunofluorescence. Flow cytometry. Western blotting. ELISA. We use an antibody that’s bound covalently to an enzyme to bind:. -antigen (direct ELISA). -antibody that is bound to antigen (indirect ELISA). Iraqi Center for Cancer and Medical Genetics Researches. Application of ELISA. Presence of antigen or the presence of antibody in a sample can be evaluated.. Determination of serum antibody concentrations in a virus test.. Dr. . Amany. . Ballow. . Consultant Immunology . Assistant professor KKUH. General Lab Director MDLAB. PhD, Diploma BSHI,MSC.. Objectives . To describe B-cells as the mediators of humoral immunity, (antibody-mediated immunity). Research Details . A vaccine-derived human antibody fragment (Fab 1A12) was crystallized alone and in complex with its target antigen, factor H binding protein (fHbp).. In a previous study, Fab 1A12 cross-reacted with fHbp variants that have different amino-acid sequences (antibodies are usually specific to one fHbp variant group)..