PDF-Pectinase Enzyme Analysis

Isopropyl or Ethyl AlcoholConcentrate Hydrochloric AcidPut 33 ml of sample in the 100 ml graduated cylinder Fill the balance of the cylinder to 100 ml with the ed Alcohol solutionFresno CA 93721Tel. Lab. South Carolina Biology Standard. 2.8. Background. Pectinases. are enzymes that breakdown the polysaccharide pectin that is located primarily in the middle lamella of cell walls. Pectin compounds are widely distributed in plant tissues- especially in fruits. . Watch the PowerPoint presentation and copy the notes.. When finished, assemble in a lab group of 2 students and begin planning your experiment.. A rough overview of your experiment is due at the end of class that includes:. Lecture 3. Objective. To understand . Specificity of enzymes. Specificity means:. Ability of an enzyme to . catalyse. a specific reaction and NO others. The active site. The . active site, . is. . C483 Spring 2013. Questions. 1. Enzymes . that join two substrates and require energy of a nucleoside triphosphate (such as ATP) to do so are called. A. ) . isomerases. .. B. ) . lyases. .. C. ) ligases.. Competitive & Non-Competitive. Learning Objectives. To learn about what enzyme inhibition is.. To learn how competitive and non-competitive inhibitors affect the active site.. To understand the implications of inhibition of rates of reaction.. Inhibition is a term used to describe the inability of a product being formed due to the presence of another substance (the inhibitor). Enzyme inhibition . can be competitive or noncompetitive. Competitive inhibition is caused when an inhibitor “competes” with the substrate in binding with the enzyme. Pratt & . Cornely. . Ch. 7. Other Factors. Other factors affect enzyme activity. Temperature. pH . pH Optimum. Determined by structural stability. Compartmentalization. Determined by active site residues. Learning Objectives. Learn how the rate of an enzyme controlled reaction is measured.. Learn how temperature affects the rate of an enzyme controlled reaction.. Learn how pH affects the rate of an enzyme controlled reaction.. 10-1 :. Munich, Anne, Fluke, and Yu. Factors Affecting . Enzyme Activity. We are doing this experiment in order to test and find out the factors that has affects on the enzyme. . The indicator tells the substance’s chemical characteristic(pH). For red cabbage indicator. Pratt & . Cornely. . Ch. 7. Enzyme Kinetics. How fast an enzyme catalyzed reaction goes. Why study enzyme kinetics?. Helps us understand mechanism of enzyme (how it works). Investigation of mutations in metabolic pathways. Pratt & . Cornely. . Ch. 7. Enzyme Kinetics. How fast an enzyme catalyzed reaction goes. Why study enzyme kinetics?. Helps us understand mechanism of enzyme (how it works). Investigation of mutations in metabolic pathways. Biology. Do not appreciate the awesomeness of enzymes!!!!. How do enzymes work?. Lock and key theory. Enzymes have a specific 3D structure. They exactly match the shape of a molecule of substrate. How do enzymes work?. astaxanthin. production. Zuharlida Tuan . Harith. , A. Chatzifragkou, D. Charalampopoulos. University of Reading. Department of Food and Nutritional Sciences. BIOREFinery. concept. 2. Bioprocessing. Therapy of enzyme defects: general considerations. How many organs are affected by the enzyme defect: One organ, a few, or all organs?. How severe is the defect?. Can the defect be adequately controlled by conventional treatment?.