PPT-Enzyme Theory

Biology Do not appreciate the awesomeness of enzymes How do enzymes work Lock and key theory Enzymes have a specific 3D structure They exactly match the shape of a molecule of substrate How do enzymes work. Watch the PowerPoint presentation and copy the notes.. When finished, assemble in a lab group of 2 students and begin planning your experiment.. A rough overview of your experiment is due at the end of class that includes:. Baseline (Flightpath D): To be able to define the terms ‘catalyst’ and ‘enzyme. ’.. Further (Flightpath C&B ): To be able to describe the properties of enzymes. To use the lock and key theory and collision theory to explain enzyme action. Protocol 10.1 through 10.3. Objective. : To cut phage genome into multiple fragments based on DNA sequence. General Introduction on Restriction Enzymes. Are also known as restriction endonucleases. Are naturally occurring enzymes used by bacteria for defensive purposes against extraneous DNA molecules. Lecturer Dr. . Kamal. E. M. . Elkahlout. Assistant Prof. of . Biotechnology. 1. CHAPTER 1. Fundamentals of Enzymes. 2. Fundamentals of enzymes. Why enzymes?. Enzyme nomenclature. Enzyme . units. Sources of . Topic 2: . Enzyme. . Kinetics. What is an Enzyme?.  . Enzymes: .  are biological molecules (proteins) that act as . catalysts*.  and help complex reactions . take place. Basically, they . break a protein down into its amino . 322 BCH. Exp. (8). In this experiment, we will continue to study . acid phosphatase . kinetics.. Objectives. To . study the effect of inhibitors on the rate of an enzymatic reaction.. To . determine the type of inhibition of acid phosphatase by inorganic phosphate and sodium fluoride. . Therapy of enzyme defects: general considerations. How many organs are affected by the enzyme defect: One organ, a few, or all organs?. How severe is the defect?. Can the defect be adequately controlled by conventional treatment?. Objectives. -Understand how Restriction Enzymes digest DNA.. -Know how to construct a . pAMP. . (plasmid) or gel.. -Given the size of fragments, gel, know how to construct a restriction map.. -Given a restriction map know how to construct a gel.. Bio-Rad Biotechnology . Explorer™ Biofuel Enzyme Kit. Instructors - Bio-Rad Curriculum and Training Specialists. Sherri Andrews, Ph.D. ., Eastern US. sherri_andrews@bio-rad.com. Damon . Tighe. , . 1 In this exercise we will look at the catalytic behavior of enzymes. You will use Excel to answer the questions in the exercise section. At the end of this session, you must hand in answers to all -. The SSERC Team. Effect of competitive and non. –. competitive inhibitors on . . ‑galactosidase. Paul Beaumont / Kate Andrews /. Margaret Louis. b. -galactosidase. b. -galactosidase. Competitive inhibition. A. . Activation Energy:. 1.. . All the reactions that proceed from initial substrates (initial state) to products (final state) consume energy. This is called free energy of the reaction.. 2.. However the substrates do not become products directly, but must be energized (absorb energy) to reach an activated or transition state. This energy is called activation energy.. 1. Enzyme concentration. 2. Temperature. 3. Hydrogen ion concentration or pH. 4. Substrate concentration. 5. Inhibitors. 6. Product concentration. 7. Activators. 8. Physical agents. Factor affecting enzyme kinetics. 1-. Describe . enzyme nomenclature. . 2-Explain the Classification of enzymes. 3-Study the. mechanizem action of enzymes. 4-illusterate factors affecting enzyme activity. . Enzyme nomenclature. Two Systems: .