PPT-Enzyme Engineering & Technology

Lecturer Dr Kamal E M Elkahlout Assistant Prof of Biotechnology 1 CHAPTER 4 Recent Advances 2 Enzymatic reactions in biphasic liquid systems E nzyme catalyzed reactions could be performed in solvents other than . C483 Spring 2013. Questions. 1. Which . statement is false about allosteric regulation? . A) It is usually the mode of regulation for the last step in reaction pathways since this step produces the final product. . Lecture 3. Objective. To understand . Specificity of enzymes. Specificity means:. Ability of an enzyme to . catalyse. a specific reaction and NO others. The active site. The . active site, . is. . Chapter 2: Section 2.5. 1. Objectives. SWBAT explain the effect of a catalyst on activation energy. . SWBAT describe how enzymes regulate chemical reactions. . and maintain homeostasis.. 2. Starter: How can this be possible. C483 Spring 2013. Questions. 1. Enzymes . that join two substrates and require energy of a nucleoside triphosphate (such as ATP) to do so are called. A. ) . isomerases. .. B. ) . lyases. .. C. ) ligases.. immobilised enzyme enzyme 21. An enzyme specifically through a this respect, lipoamide dehydrogenase particularly useful enzyme comprises two polypeptide chains each binding binding and containing 10 Lecturer Dr. . Kamal. E. M. . Elkahlout. Assistant Prof. of . Biotechnology. 1. CHAPTER 3. Immobilized . enzymes and their . uses. 2. Enzyme reactors. An enzyme reactor consists of a vessel, or series of vessels, used to perform a desired conversion by enzymatic means (Figure 5.1). . Inhibition is a term used to describe the inability of a product being formed due to the presence of another substance (the inhibitor). Enzyme inhibition . can be competitive or noncompetitive. Competitive inhibition is caused when an inhibitor “competes” with the substrate in binding with the enzyme. Pratt & . Cornely. . Ch. 7. Other Factors. Other factors affect enzyme activity. Temperature. pH . pH Optimum. Determined by structural stability. Compartmentalization. Determined by active site residues. Learning Objectives. Learn how the rate of an enzyme controlled reaction is measured.. Learn how temperature affects the rate of an enzyme controlled reaction.. Learn how pH affects the rate of an enzyme controlled reaction.. 10-1 :. Munich, Anne, Fluke, and Yu. Factors Affecting . Enzyme Activity. We are doing this experiment in order to test and find out the factors that has affects on the enzyme. . The indicator tells the substance’s chemical characteristic(pH). For red cabbage indicator. Phosphorylation is a type of covalent modification that activates or deactivates an enzyme.. a. ) A . kinase activates an . inactive enzyme . by phosphorylation. . b. ) A . phosphatase activates . an inactive . Objectives. To monitor the progress of an enzyme catalyzed reaction (Acid phosphatase).. To determine the initial rate of the reaction (. V. i. ).. Important terms and points:. Enzyme kinetics. The central approach for studying the mechanism of an enzyme-catalyzed reaction is to study enzyme kinetics. . 322 BCH. Exp. (8). In this experiment, we will continue to study . acid phosphatase . kinetics.. Objectives. To . study the effect of inhibitors on the rate of an enzymatic reaction.. To . determine the type of inhibition of acid phosphatase by inorganic phosphate and sodium fluoride. . Theories of Enzyme Evolution. Overview. (1) . Divergent. . retrograde. evolution, recruiting . adjacent enzymes in pathway. and constrained by . binding similar molecules . as substrates or products..