Vrushank Dharmesh Bhatt Moussa Diolombi Monika Haack Janet Mullington Department of Neurology Beth Israel Deaconess Medical Center and Harvard Medical School Boston MA 02215 ID: 1011829
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1. EFFECT OF REPEATED EXPOSURE TO SLEEP RESTRICTION ON INTERLEUKIN 6 LEVELS IN HUMANSVrushank Dharmesh Bhatt, Moussa Diolombi, Monika Haack, Janet MullingtonDepartment of Neurology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215Interleukin-6 (IL-6) is a pleiotropic cytokine produced by numerous types of immune and nonimmune cells and is involved in many pathophysiologic mechanisms in humans.Previous studies have suggested that IL-6 is a putative ‘sleep factor’ and its circadian secretion correlates with sleep/sleepiness. Short periods of experimental sleep restriction have been shown to elevate IL-6, which has been suggested to increase the risk for cardiometabolic diseases. However, the effect of repeated exposure to sleep restriction on IL-6 is unknown.INTRODUCTIONSTUDY DESIGNFigure 1: IL-6 levels DISCUSSIONFUTURE DIRECTIONSProgram #615.7METHODSStudy Design: Participants were randomized to a 22-day study of repeated cycles of sleep restriction (4hr sleep/night for 3 nights followed by one recovery sleep, 4 cycles) or routine sleep (8hr sleep/night), following two nights of baseline sleep. Blood Sampling: Blood was sampled from fasting recumbent participants in the morning after awakening, during baseline, third day of each sleep restriction or control cycle, and following recovery. Total- 13 blood drawsIL-6 Measurement: Plasma concentrations of IL-6 were measured using high sensitivity immunosorbent assay (R&D Systems ELISA), CV<15%.Study Environment: Participants remained in the Clinical Research Center for the duration of study, with controlled fluid and calorie intake, with normal interaction and physical activity.Figure 2: The sleep restriction group showed a significant increase in IL-6 levels in the morning during the first sleep restriction cycle, exceeding control levels by 20% (p<0.05). These levels did not return to baseline in the remaining sleep restriction cycles when compared with the control group. Though there was a slight increase in the IL-6 levels in the sleep restriction group in the night after the first sleep restriction cycle, this increase was not significant as compared to the baseline levels. These data suggest that a good night's sleep is associated with decreased daytime secretion of IL-6 and a decreased exposure of tissues to this pro-inflammatory cytokine and potentially detrimental actions of IL-6. IL-6 levels return to baseline levels after recovery sleep.ACKNOWLEDGEMENTREFERENCESThis work was supported by science research funds from National Institute of Health (HL-106782) and Harvard Catalyst CTSC NIH UL1 TR001102.According to recent findings, IL-6 levels are associated with increased mortality in elderly. So the possibility that anti-inflammatory therapies might provide a new approach to cardiovascular disease treatment and prevention.Other inflammation markers will be analyzed to elucidate the exact mechanism for understanding the potential association of these cytokines with sleep, health and well-being.RESULTSVariableControlSleep RestrictionWomen/Men11/1111/10Age, years31+231+2BMI, kg/m224+124+1Habitual Sleep, h7.8+0.17.8+0.1 Sleep Period Sleep period with intensive physiological monitoring Blood Draw Urine collection (morning) Urine collection (night) 24 hr periods with PSG12345