Dr Mah Jabeen Muneera Associate Professor Department of Anatomy KEMU LAMINA PROPRIA Connective tissue upon which epithelium rests Support provide nutrition epithelium is avascular ID: 459645
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Basal Lamina & Basement Membrane
Dr. Mah Jabeen MuneeraAssociate ProfessorDepartment of AnatomyKEMUSlide2Slide3
LAMINA PROPRIA
Connective tissue upon which epithelium restsSupport, provide nutrition, epithelium is avascularArea of contact is increased by folds BASEMENT MEMBRANESheet of ECM intervenes between epithelium and lamina
propria
Source
Function
Special stain (PAS)
Also present around glial cells, adipocytes & muscle cells
Not present around other CT cellsSlide4
Basal lamina
Evident by routine EM preparationComposed of upper LAMINA LUCIDA (glycoproteins)LAMINA DENSA on lower aspectSlide5
Components
LamininGlycoproteinsAssociated with integrinsType IV collagen, XV, XVIII, VIIEntactin (Nidogen
) &
Perlecan
,
Agrin
Link the first two components
Proteoglycans ; hydrated,
negative charge controls ionsSlide6
Tissue preparation by HPF method
HPF- high pressure freezingOnly lamina densa is presentLamina lucida is considered to be an artifactSlide7
External lamina
In non epithelial cellsSlide8
Formation of basal lamina
Self assembly of collagen type IV & LamininCalcium dependent polymerization Slide9
RETICULAR LAMINA
FUNCTIONS:SupportBarrierInfluence cellular activity e.g. proliferation, differentiation BASEMENT MEMBRANEPAS +ve layer Visible by Light MicroscopeTwo basal laminae
One basal lamina & one reticular laminaSlide10Slide11
Attachment of basal lamina to underlying tissue
Anchoring fibrilsMutations-dystrophic epidermolysis bullosaFibrillin microfibrilsMarfan’s syndrome
Projections of basal laminaSlide12
Functions
Regulator of cell behaviorOrgan specific molecules Structural attachmentCompartmentalizationFiltrationTissue Scafolding (barrier against tumor cell invasion)Regulation & signaling