PPT-What is a Stain A stain is a substance that adheres to a cell, giving the cell color

The presence of color gives the cells significant contrast so are much more visible Different stains have different affinities for different organisms or different parts of organisms They are used to differentiate different types of organisms or to view specific parts of organisms. Gram Stain Reaction. The Gram stain reaction, named after the Danish bacteriologist Hans Christian Gram, was originally devised in 1882.. Gram staining is based on the ability of the bacterial cell wall to retain the crystal violet dye. . Cheng . Zhang. Thurs 1 Dec 11. MM Tutorial. Gram staining. Fixed film (heat kill bacteria etc...). Methyl violet . Lugol’s. /gram’s iodine. Decolourise with acetone. Methyl red .  . STAIN 2 (counter stain): pink-red colour. A stain is a substance that adheres to a cell, giving the cell color. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . Gram stain . Ziehl. . Neelsen. stain. . Requires the use of at least 3 chemical reagents.. Primary Stain. : . stain all the cells.. Decolorizing agent: . may or may not remove the primary stain from the entire the cell or only from certain cell structure. . Clinical Bacteriology II. CLS 413. Mrs. . Deemah. . Dabbagh. CLS Department. College of Applied Medical Sciences. King Saud University. Course Content. Enterobacteriacae. Lactose Fermenters:. Escherichia coli. BIOLOGY & BIOTECHNOLODY DEPARTMENT. In our laboratory, . bacterial morphology . (form and structure) may be examined in two ways:. By observing . living unstained organisms. (. wet mount. ).. 2. By . A stain is a substance that adheres to a cell, giving the cell color. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . Biology and Biotechnology department. Differential . stain separate bacteria into group . . Gram . Stain :. Differentiate bacteria into 2 groups:. Gram . positive bacteria . Gram . negative bacteria . Gram stain . Ziehl. . Neelsen. stain. . Requires the use of at least 3 chemical reagents.. Primary Stain. : . stain all the cells.. Decolorizing agent: . may or may not remove the primary stain from the entire the cell or only from certain cell structure. . Only use oil on 100x objective lens. Never get it onto any of the other objectives! . Never use the coarse adjustment with the 40x and 100x objectives.. . Ex. . 3: Staining Techniques - Gram Stain, part 1. Differentiate bacteria into two large groups (the Gram Positive and the Gram negative).. Gram status is important in medicine; the presence or absence of a cell wall will change the bacterium's susceptibility to some antibiotics. tissues, cells, cell components, cell contents, or microorganisms.. *The stain may be . natural or synthetic. . . *The object stained is called the . substrate. . . *Staining facilitates the observation of a substrate by introducing . refractile. body within the vegetative cells. Procedure: (fig.10) . 1- Prepare a fixed smear.. 2- Flood the smear with 5% malachite green and let the stain react for 1 minute .Using your . bunsen. burner, heat the top of the stained slide until steam rises but do not let it boil. This step is performed by passing the burner back and over the slide for about 5 minutes.. Bacterial Smear Preparation. It is a preparation that includes a . small amount of biological sample or culture . spread. . in a very thin film on the surface of the slide. . . Smear preparation is a step before staining..