Quantal analysis Indexing synaptic transmission a number The neuromuscular junction Whiteboard work NMJ history of quantal synaptic transmission Katz 1954 Mean quantal content of failures0 of singles1 of doubles2 ID: 578641
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Slide1
Synaptic transmission
Quantal analysis
Indexing synaptic transmission (a number)Slide2
The
neuromuscular junctionSlide3Slide4Slide5
Whiteboard work- NMJ history of quantal synaptic transmission. Katz 1954.Slide6
Mean quantal content =
∑ (# of failures)(0) + (# of singles)(1)+ (# of doubles)(2)…
Total number of stimulus trials
Mean quantal content =
Mean amplitude (or area) of evoked quantal events
Mean amplitude (or area) of spontaneous quantal eventsSlide7
Minis
Some evidence for distinct differences from evoked and spontaneous release pools.
Chung et al. 2010 -
hippocampal
GABAergic inhibitory synapses.
Fredj & Burrone, 2009 - rat hippocampal neurons.
Sara et al., 2005 - rat hippocampal neurons.
Controversial (Wilhelm et al., 2010 ... same pool )Slide8Slide9
POSTSYNAPTIC
PRESYNAPTICSlide10
POSTSYNAPTIC
PRESYNAPTIC
APSlide11
Not saturatedSlide12
SaturatedSlide13
Not saturated
Off centerSlide14
Saturated due to
smaller synapse
With same amount
of transmitter
Not saturatedSlide15
2
Not saturated to
saturated based on
vesicle size and amount
released.
1
3
1
2
3Slide16
Not saturated
but not enough time
to recycle and refill
TimeSlide17
Not saturated
but not enough time
to recycle and refill
but can recruit from RP
RP
Time
RPSlide18
Not saturated
but increase density
of postsynaptic receptor
density
Time
Test ?Slide19
Case 1:
Case 2:
Focal recording
Intracellular muscle
recording
Missing quanta
muscle
nerveSlide20Slide21Slide22
http://en.wikipedia.org/wiki/Purkinje_cell#mediaviewer/File:Gray706.pngSlide23
http://etc.usf.edu/clipart/53100/53184/53184_purkinje.htmSlide24
Classical
Quantal
Counting Methods vs. New Stochastic Measurements.
More accurately measure
n (number of docked sites)
and
p (probability of fusion at a site).
Find subsets of quantal events.Slide25
Citizen science with high school students and adults from around the world participating in analysis of synaptic transmission
http://web.as.uky.edu/Biology/faculty/cooper/Citizen%20Science%20folder/CitizenScienceProjectPage.htm