and the usage of them in antiviral immunity Heleri Kirsip Bioinformaatika JC 10112016 Mis on endogeenne viiruslik element EVE Endogeensed viiruslikud elemendid viiruse g enoom ID: 909065
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Slide1
Endogenous bornavirus elements in eukaryotesand the usage of them in antiviral immunity
Heleri Kirsip
Bioinformaatika
JC
10.11.2016
Slide2Slide3Mis on endogeenne viiruslik
element (EVE)?
Endogeensed viiruslikud elemendid – viiruse g
enoom
,
geenid
või
geenide
tükid
, mis on integreerunud organismi genoomi ning fikseerunud põlvkondade jooksul (
Bejarno
et
al
.
, 1996).
Slide4Endogenous bornavirus-like elements
(-)ssRNA,
Mononegavirales
,
Bornaviridae
EBLs (endogenous bornavirus-like elements)
Nucleoprotein
EBLN
Via LINE-1 element
Borna
disease virus (BDV)
Antiviral immunity with EBLN elements
Slide5Slide6Katzourakis
and Gifford, 2010
Slide7Katzourakis
and Gifford, 2010
Slide8Slide9itEBLNs and hsEBLNs
Ictidomys
tridecemlineatus
(TLS)
Intact ORF
77%
aminoacid
identity
Predicted transcripts present in several tissue samples from both breeding and wild-captured TLSs by RT-PCR
Human
Intact ORF
41%
aminoacid
identity
Transcripts are expressed as RNA in several tissues
Slide10itEBLNs and hsEBLNs
itEBLN
Co-localizes with the viral factory in the nucleus
Decreases exogenous BDV infection and replication
Incorporated into the viral ribonucleoproteins
hsEBLN
Localizes in the cytoplasm
Exogenous BDV infection and replication remains the same
Is not incorporated into viral ribonucleoproteins
Slide11Slide12Slide13Dominant negative forms of viral
proteins
.
Slide14Transcript reversion with anamnestic piRNA silencing (TRAPS)
hsEBLN
3 within annotated
piRNA
clusters
*hsEBLN-7 –
piRNA
like RNA (shorter)
Marmoset EBLN
1 within annotated
piRNA
clusters
*cjEBLN-7 –
piRNA
like RNA (shorter)
mmEBLN
/
rnEBLN
2 (3) within annotated
piRNA
clusters
Slide15Transcript reversion with anamnestic piRNA silencing (TRAPS)
Mus
musculus
(
mmEBLN
)
Expressed as RNA in at least one tissue (all 7 human EBLN elements, 2
mmEBLN
)
Within PIWI-interacting RNA (
piRNA
)-generating loci – “
piRNA
clusters”
EBLNs expressed as
piRNAs
EBLN-derives
piRNAs
mapped to the antisense strand relative to bornavirus N mRNA
Post-transcriptionally silencing
Parrish
et al
., 2015
Slide16Transcript reversion with anamnestic piRNA silencing (
TRAPS
)
Slide17lncRNA immunity
Human (
hsEBLN
)
hsEBLN
may affect the expression of its neighbouring COMMD3 gene (downregulated)
Protein locates in cytoplasm – long non-coding RNA (lncRNA)
COMMD3 – NF
κ
B pathway – counteract invading pathogens
Slide18Slide19Thank you!