PDF-Timing and Sequencing of

The Correctional ProgrammingGrant Duwe PhDDirector Research and EvaluationApril 2017Email grantduwestatemnusDisclaimer The analyses and conclusions presented here are those of the author and should no. Dan . Russell. Overview. Prologue: Assembly and Finishing. The Past: Sanger. The Present: Next-Gen (454, . Illumina. , …). The Future: ? (. Nanopore. , . MinION. , Single-molecule). Overview. Prologue: Assembly and Finishing. Dan . Russell. The past, present, and future of DNA . sequencing*. Dan . Russell. *DNA sequencing:. D. etermining the number and order of nucleotides that make up a given molecule of DNA.. (Relevant) Trivia. Craig A. . Praul. Co- Director . Genomics Core Facility. Huck Institutes of the Life Sciences. Penn State University. A very short history of DNA sequencing. I started from the conviction that, if different DNA species exhibited . Objectives. After completing this module, you will be able to:. Describe a flow for obtaining timing closure. Interpret a timing report and determine the cause of timing errors. Apply Timing Analyzer report options to create customized timing reports. Making . Your Applications Virtual . Chris Green. Program Manager. Microsoft Corporation. SESSION CODE: . VIR320. Required Slide. Session Objectives And Takeaways. Understand the sequencing process. See best practices for . - . INTRODUCTION. - SANGER DIDEOXY METHOD. - AUTOMATED SEQUENCING. - NEXT. GENERATION OF SEQUENCING METHODS. MISS NUR SHALENA SOFIAN. INTRODUCTION. 1977:. . Frederick Sanger along with Allan . Maxam. - . INTRODUCTION. - SANGER DIDEOXY METHOD. - AUTOMATED SEQUENCING. - NEXT. GENERATION OF SEQUENCING METHODS. MISS NUR SHALENA SOFIAN. INTRODUCTION. 1977:. . Frederick Sanger along with Allan . Maxam. Laser / RF Timing ( E ngineering of Femtosecond Timing Systems) Josef Frisch SLAC Femtoseconds Bryan Bandli , Scanning Electron Microscopy Laboratory, University of Minnesota 70fs Eye blink ½ human hair How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. TGATTTTAAAAAAATATT…. mRNA. RNA-SEQ. TruSeq. . Stranded. . mRNA. RNA-SEQ. The first . step. in the . workflow. . involves. . purifying. the . poly-A. . containing. . mRNA. . molecules. . using. . poly-T. . oligo. Hardison. Genomics 3_1. 1. 1/20/14. nucleo. s. ides. A nucleo. t. ide has one or more phosphates attached to the 5’ hydroxyl of the (deoxy)ribose. Building blocks of DNA. 1/20/14. 2. Structure of a dinucleotide. Adapted . from:. http. ://. ase.tufts.edu/chemistry/walt/sepa/geneticsofrace.html. Uploaded: January 8, 2017. Genetics of Race. Lesson 1: . Introduction. Goals:. Introduce module topic . Provide necessary background. Dr. . Sudha. . Kumari. Assistant Professor. Department of Veterinary Microbiology. Bihar Animal Sciences University, Patna. INTRODUCTION . The . The Sanger method. DNA sequencing is the primary method for gene and protein characterization. . (protein sequence can be predicted from DNA sequence). . https://. www.illumina.com. /techniques/sequencing/.