PPT-Inhibition of PRMT5 leads to MAPK/ERK-driven upregulation of EBV lytic protein BZLF1 and

Taylor Cash Rachel Saadey Polina Shindiapina The Ohio State Universitys Autumn Undergraduate Research Festival November 2021 Introduction Over 90 of the human population has been identified as seropositive for EpsteinBarr Virus EBV . 1 Competitive Inhibition Fig 8- 2 Competitive InhibitionCTEuli S + S E P + E c = K . ( [ c / K c / Vx sl re S bu i n a S bd t fee E a a s wre S bd c w S f fee E S Pratt & . Cornely. . Ch. 7. Other Factors. Other factors affect enzyme activity. Temperature. pH . pH Optimum. Determined by structural stability. Compartmentalization. Determined by active site residues. Pratt & . Cornely. . Ch. 7. Enzyme Kinetics. How fast an enzyme catalyzed reaction goes. Why study enzyme kinetics?. Helps us understand mechanism of enzyme (how it works). Investigation of mutations in metabolic pathways. Index. Introduction. Discovery. Linkage data from transduction. Specialized transduction. Lytic and Lysogenic Cycle. Introduction. Transduction is the process by which DNA. . is transferred from one bacterium to another by a virus.. simpler. Ex: the flu. A virus with this type of cycle makes you sick right away. What about the other type of life cycle?. The . Lysogenic. Cycle. The . lysogenic. cycle is slower and more complex. apsid. N. ucleic Acid. R. eceptors. DNA or RNA. Membrane around virus. Proteins that help virus get into proper host. Attachment. The virus attaches to receptors on the host cell.. The virus is able to get into the cell in one of three ways.. Peter Congdon, Queen Mary University of London, School of Geography & Life Sciences Institute. Outline. Background. Bayesian approaches: advantages/cautions. Bayesian Computing, Illustrative . BUGS model, Normal Linear . In lytic cycle, the phage DNA enters the host bacterium, its genes are transcribed, the phage GM replicated, and the protein components of the phage particle are produced. . Finally, the host bacterium is lysed to release the assembled progeny particles by the process of lysis.. . . . Pathogenesis of EBV Infection. . Cohen . NEJM 2000. Early IM: NK cells. non-HLA specific CTLs. Late IM: HLA-restricted CTLs (CD8 and CD4):. Lytic epitopes - up to 40% of CD8 cells . By: Maddy Connell, Ava . Taurino. , and Shane Landue. Overview .  What is a virus?.  Structures of viruses.  How viruses reproduce.  Cells infected by viruses.  Diseases caused by RNA viruses.  The Lytic Cycle. Frances A. Rosario FNP-S. Suny Poly. Epstein Barr Virus (EBV). Epstein-Barr Virus is a herpesvirus that is transmitted via intimate contact between at risk individuals and asymptomatic EBV shedders. EBV is the primary agent in pts with . .. Learning Goal . . Describe competitive and . noncompetitive inhibition . and reversible and irreversible inhibition. .. Inhibitors. Inhibitors. are molecules that cause a loss of catalytic activity.. 1. , Shelby Sloan. 1. , Kris . Vaddi. PhD. 2. , Rosa . Lapalombella. PhD. 1. , . Jihye. Paik PhD. 3. , and Robert A. . Baiocchi. MD PhD. 1. et al. 1 . Division of Hematology, Department of Internal Medicine, Ohio State University Wexner Medical Center, Columbus, OH, USA . A Pediatric Transplant Infectious Diseases . Learning Module. Using the interactive modules. The basic modules are designed . to take ~ 30 minutes to complete. , especially if you choose to investigate all of the .