PPT-Phage Strategies Some phages have only a single strategy for survival on infecting a susceptible

In lytic cycle the phage DNA enters the host bacterium its genes are transcribed the phage GM replicated and the protein components of the phage particle are produced Finally the host bacterium is lysed to release the assembled progeny particles by the process of lysis. Rhodococcus. . erythropolis.  phages were isolated from northeast Louisiana area soil samples.  To date, . Rhodoccocus. phages Chewy VIII and Trina have been sequenced (Illumina Sequencing, University of Pittsburgh) and their genomes annotated with the DNA Master bioinformatics platform.  Chewy VIII is a . as a novel approach to . Biofilm. inhibition and remove. Azizian. . Reza1, . Azizi. . Jalilian. . Farid2.*, . Askari. . Hasan1, . Naser. . Ahmad1, . Karimi. . Sajedeh1, . Sadeghifard. . Norkhoda1, . Khansa. . Bukhari. Maryum. . Zeb. . History and Definition. Frederick . Twort. (1915) and Felix . d'Herelle. (1917) were the first to recognize bacteriophage.. In the 1930s and after words virologists utilized these viruses as model systems to investigate many aspects of virology. Yvonne. . Lin. . AP. . Bio. . 2. a.k.a. Phages. Bacterial . VIRUS. Attacks, infects, and replicates within bacteria. Discovered. . by. . Frederick. . Twort. . (Great. . Britain, 1915). Species specific – named after the group or species it infects. . . Prepare phage for testing. *Create high titer lysate via flooding method.. *Isolate phage DNA and replicate using PCR.. *Send off isolated phage to HHMI facility for . Series I: . Isolation and Purification of Phage. 1. We created an enrichment culture in order to grow bacteriophages and ultimately isolate a selected phage to examine. . 2. We generated a plaque assay using . Gordonia terrae. phages reveals novel aspects of lysis, virion morphology, and host range. Matthew Benczkowski, Melanie Umbaugh, Pitt SEA-PHAGES 2015-16 students, Rebecca Bortz, Emily Furbee, Sarah Grubb, Deborah Jacobs-Sera, Matthew Montgomery, Marcie Warner, Welkin Pope, and Graham Hatfull. Abdulrahman. . Alazemi. , . Shahroze. Abbas, Liam Lewis, Donald Ta, Ann Vo. Overview. Clustered repeats. Wide variety of functions. History largely unknown. Regulatory Sites. A segment capable of altering expression of specific genes. By Monique Hunter. Bacteriophages or phages are viruses that infect bacteria.. In 1917, . French-canadian. microbiologist Felix . d’Herelle. observed an unknown substance killing bacteria as did his . Monique L. Mills. , Judd Bragg,  Asri Bruce, Ari Dehn, Morgan Hefner, Dylan Katon, Dustin McHugh, Payton Petersen, Franck Zeba, . Gerard P. Zegers, Shallee T. Page.. University of Maine at Machias, Machias, ME 04655. World’s Largest provider of commercial bacteriophage products for agricultural use.. Bacteriophage?. Bacteria-eater. Naturally occurring bacterial virus. Cannot infect mammalian, fungal, yeasts or molds. 24. ) structure for Osiris gp52 superimposed using Cn3D (. 32. ) on the phage P22 c2 repressor protein reveals high structural homology, with an altered C-terminal tail. Osiris gp52 is similar. . to P22’s c2 repressor near the N-terminus that is the DNA binding domain as predicted by . . DNA in mycobacterial genomes. . Bobby Chaggar. BNFO 301. Lysogeny. Group. Compare (SEQUENCE-SIMILAR-TO) each lysogenic, . mycobacteriophage. to a list of all mycobacteria in . BioBIKE. (. PhAnToMe. HHMI Logistics. . Name badges must be worn at all times. Restrooms. No roaming through the building. Questions? – Ask . us. Refreshments . (keep to the schedule). Technology. Live Streaming. The microphones.