PPT-Amplification and Assemblage of Phage

Josiah Austin Julia Tang Tiffany Nguyen Michael Ketteh Lynn Zhou Dr Mao PhD The M13 phage is a humanfriendly bacteriophage that only infects bacteria cells 1 Its a nanofibershaped phage with five different coating proteins wrapped around its DNA Figure 1b The M13 phage can be engineered to display the exogenous peptides on its surface This phage display technique allows us to study the function of short peptides that are derived from the cells growth factors or proteins Additionally M13 phage have the ability to selfassemble into organized films that look like which can serve as a matrix for a cell culture The phage film matrix can give us a better understanding of the role of short peptides in growth factors The traditional method of making phage films requires the use of a pipette to drop the negatively charged phage solution onto the polylysine Figure 2 However manually the results are not replicable and the process is time consuming In order to make more organized and repeatable phage films we use a programmable slide stainer machine Carl Zeiss Inc that can dip the slides into a each solution allowing time to dry and giving it the ability to agitate Resultantly we built phage films while varying soaking time phage concentration and the number of phage layers to determine the best conditions to form wellordered phage films with the help of slide stainer. Nick Fox, University of Sheffield, UK . Pam . Alldred. , Brunel University, UK . Introduction . Rationale for the paper.. Anthropocentrism and sexuality.. Anti-humanism and sexuality.. Study of young men’s sexuality.. 1. Définition de l’assemblage.  . Le choix d’une solution d’assemblage dépend . du.  : .  . Les . procédeés. d’assemblages . s. e . classent en 3 . familles:.  . Un . assemblage mécanique peut être. Objective: You will analyze and describe artworks in order to define and apply the ideas of assemblage and formalism to your abstract non-objective artworks.. DRILL: . Get a yellow Final Exam Review sheet.. LOCK. it out on the first move. HI, DROXYLATE and . SWEET RELEASE defeat METHYLATE. but stay in play until cancelled. If host plays RESTRICT. then phage can counter with. METHYLATE – move both cards to the discard pile. . . Prepare phage for testing. *Create high titer lysate via flooding method.. *Isolate phage DNA and replicate using PCR.. *Send off isolated phage to HHMI facility for . of . Genomic . DNA Fragments. OrR. Amplification. To get particular DNA in large amount. Fragment size shouldn’t be too long. The nucleotide sequence at each end is known.. How to get known sequence from both end of particular genome fragment?. How to identify circularly permuted genomes, physical ends, 3’ overhangs, terminal repeats, and nicks.. Circularly Permuted Genomes. Some phages have circularly permuted genomes. This means a linear concatamer of phage DNA is synthesized, used to fill a phage head, then cut when the head is full. Generally, one head will fit more than 100% of a genome, say, 103-110%. This ensures that wherever the DNA is cut, at least one working copy of each gene is present.. Michael Woods. w. ith Jesse . Heley. , Francesca . Fois. , Laura Jones, . Anthonia. . Onyeahialam. , Samantha Saville, Marc Welsh. Aberystwyth University, UK. International Symposium on Assemblage Thinking – Mytilene, June 2017. What is. Assemblage. ?. •Assemblage sculpture is the bonding of. shapes or objects by gluing, soldering,. pasting, or nailing. These objects are. mainly found objects (Katz 175).. • Basically, assemblage is a 3-dimensional. Michael woods. Aberystwyth University. m.woods@aber.ac.uk. Twitter: @. globalrural. IGU Commission on the Sustainability of Rural Systems Annual Colloquium. Lisbon and Porto . – July 2015. Globalization is not a steamroller. World’s Largest provider of commercial bacteriophage products for agricultural use.. Bacteriophage?. Bacteria-eater. Naturally occurring bacterial virus. Cannot infect mammalian, fungal, yeasts or molds. Goal: Reactions where the product grows exponentially with the number of cycles.. Main classes of methods:. (1) Thermal-Cycling: PCR. (2) Isothermal: many methods. Note: If RNA is to be detected, usually first transform to DNA. or phages are viruses that specifically infect bacteria. . Viral vectors are those in which gene of interest is incorporated in the genome of virus.. The phage particle attaches to the outer surface of bacterium and injects its DNA into the cell.. Dan Russell. Pauline: 7?!. Happy. Sad. 19. 2. Failure. Success. 19. 41. Star Trek. Star Wars. 9. 17. Batman. Superman. 8. 4. Mulan. Belle. 4. 4. Rapper. Scientist. 37. 43. 3. 5. 6. 10. 0. Mother. Father.