PPT-Isolate phage

Prepare phage for testing Create high titer lysate via flooding method Isolate phage DNA and replicate using PCR Send off isolated phage to HHMI facility for . Geobacillus. and . Heamophilious. . Phages. Ramey . Elsarrag. Introduction. Tandem repeats are adjacently repeated patterns of DNA sequences. They are associated with genetic instability and are used for forensic identification. Khansa. . Bukhari. Maryum. . Zeb. . History and Definition. Frederick . Twort. (1915) and Felix . d'Herelle. (1917) were the first to recognize bacteriophage.. In the 1930s and after words virologists utilized these viruses as model systems to investigate many aspects of virology. Very complex shape, requiring 20 gene products for assembly. - . Capsid. (head), contains linear dsDNA genome. - . Tail. , consists of sheath plus internal tube. Bacteriophage T4. Figure 11.2. Phage T4 binds to . 9/30/2010. The role of Artificial Neural Networks in Phage Research . What is an Artificial Neural Network?. Mathematical and computational model. Motivated by biological neurons. Trained by using features to learn patterns and commonalities. Gary Larson – The Far Side. ASSESSMENT. UCLA Instructional . Laboratories: Where Leading-Edge . Science Education . Is Always Bruin. Jordan Moberg Parker. Research Immersion Lab in Virology. http://www.youtube.com/watch?v=rzdwfwuVWUU. Yang Xin. Paper presentation. PNAS 2009, 106(12):4629-4634. Introduction. Antimicrobial drug development is increasingly lagging behind the evolution of antibiotic resistance. as a result, there is a pressing need for new antibacterial therapies that can be readily designed. Abdulrahman. . Alazemi. , . Shahroze. Abbas, Liam Lewis, Donald Ta, Ann Vo. Overview. Clustered repeats. Wide variety of functions. History largely unknown. Regulatory Sites. A segment capable of altering expression of specific genes. Protocol 10.1 through 10.3. Objective. : To cut phage genome into multiple fragments based on DNA sequence. General Introduction on Restriction Enzymes. Are also known as restriction endonucleases. Are naturally occurring enzymes used by bacteria for defensive purposes against extraneous DNA molecules. . Josiah Austin, Julia Tang, Tiffany Nguyen, Michael . Ketteh. , Lynn Zhou, Dr. Mao PhD. The M13 phage is a human-friendly bacteriophage that only infects bacteria cells. 1. . It’s a nanofiber-shaped phage with five different coating proteins wrapped around its DNA (Figure 1b). The M13 phage can be engineered to display the exogenous peptides on its surface. This phage display technique allows us to study the function of short peptides that are derived from the cell’s growth factors, or proteins. Additionally, M13 phage have the ability to self-assemble into organized films that look like which can serve as a matrix for a cell culture. The phage film matrix can give us a better understanding of the role of short peptides in growth factors. The traditional method of making phage films requires the use of a pipette to drop the negatively charged phage solution onto the poly-lysine (Figure 2). However, manually, the results are not replicable and the process is time consuming. In order to make more organized and repeatable phage films we use a programmable slide stainer machine (Carl Zeiss, Inc.) that can dip the slides into a each solution allowing time to dry and giving it the ability to agitate. Resultantly, we built phage films while varying soaking time, phage concentration, and the number of phage layers to determine the best conditions to form well-ordered phage films with the help of slide stainer.. Yang Xin. Paper presentation. PNAS 2009, 106(12):4629-4634. Introduction. Antimicrobial drug development is increasingly lagging behind the evolution of antibiotic resistance. as a result, there is a pressing need for new antibacterial therapies that can be readily designed. World’s Largest provider of commercial bacteriophage products for agricultural use.. Bacteriophage?. Bacteria-eater. Naturally occurring bacterial virus. Cannot infect mammalian, fungal, yeasts or molds. Circularly Permuted Genomes. Some phages have circularly permuted genomes. This means a linear concatamer of phage DNA is synthesized, used to fill a phage head, then cut when the head is full. Generally, one head will fit more than 100% of a genome, say, 103-110%. This ensures that wherever the DNA is cut, at least one working copy of each gene is present.. NCBI Pathogen Detection . https://www.ncbi.nlm.nih.gov/pathogens. At a glance. Use the Pathogen Isolates Browser to locate your isolate. https://www.ncbi.nlm.nih.gov/pathogens. Check the exceptions report. Dan Russell. Pauline: 7?!. Happy. Sad. 19. 2. Failure. Success. 19. 41. Star Trek. Star Wars. 9. 17. Batman. Superman. 8. 4. Mulan. Belle. 4. 4. Rapper. Scientist. 37. 43. 3. 5. 6. 10. 0. Mother. Father.