PPT-Mycobacteriophage discovery: potential applications for phage therapy

of mycobacterial disease Fernanda Alonzo Adebanjo Adedoja Ann M Findley School of Sciences ULM Chris R Gissendanner School of Pharmacy ULM Abstract Nontuberculous . LOCK. it out on the first move. HI, DROXYLATE and . SWEET RELEASE defeat METHYLATE. but stay in play until cancelled. If host plays RESTRICT. then phage can counter with. METHYLATE – move both cards to the discard pile. . discovery: potential applications for phage therapy . of . mycobacterial disease. Fernanda . Alonzo, . Adebanjo. . Adedoja. , . Ann . M. Findley, School of Sciences, . ULM; . Chris . R. . Gissendanner. Panelists:. Ky Fullerton, Umpqua Bank. Anton Litchfield, Encore Discovery Solutions. Josh Sasaki, Miller Nash LLP. Moderator: . Jovita Wang, Miller Nash LLP. Sponsored by Miller Nash LLP. Page . 2. Failure to Plan is Planning to Fail. . . Prepare phage for testing. *Create high titer lysate via flooding method.. *Isolate phage DNA and replicate using PCR.. *Send off isolated phage to HHMI facility for . David Hussey, PhD. Case Western Reserve University, Cleveland, Ohio. Anne M. Reed NMT, MT-BC. Beech Brook, Cleveland, Ohio. Create the Next Wave: American Adoption Congress and Adoption Network Conference, April 12, 2013 . How to identify circularly permuted genomes, physical ends, 3’ overhangs, terminal repeats, and nicks.. Circularly Permuted Genomes. Some phages have circularly permuted genomes. This means a linear concatamer of phage DNA is synthesized, used to fill a phage head, then cut when the head is full. Generally, one head will fit more than 100% of a genome, say, 103-110%. This ensures that wherever the DNA is cut, at least one working copy of each gene is present.. Categories of Net Applications. Discovery, Communication, Collaboration. Web 2.0. E-Learning and Telecommuting. Net Applications 1: Discovery. Search engines. General- or special-purpose. Metasearch (aggregator). Also, palindromes. What are they?. Short sequences (between 4 and 13-ish nucleotides) – one might even say they’re very short. Occur multiple times in the genome, at . dispersed. intervals (not repeated right next to each other) – “distributed or spread over a wide interval”. . Josiah Austin, Julia Tang, Tiffany Nguyen, Michael . Ketteh. , Lynn Zhou, Dr. Mao PhD. The M13 phage is a human-friendly bacteriophage that only infects bacteria cells. 1. . It’s a nanofiber-shaped phage with five different coating proteins wrapped around its DNA (Figure 1b). The M13 phage can be engineered to display the exogenous peptides on its surface. This phage display technique allows us to study the function of short peptides that are derived from the cell’s growth factors, or proteins. Additionally, M13 phage have the ability to self-assemble into organized films that look like which can serve as a matrix for a cell culture. The phage film matrix can give us a better understanding of the role of short peptides in growth factors. The traditional method of making phage films requires the use of a pipette to drop the negatively charged phage solution onto the poly-lysine (Figure 2). However, manually, the results are not replicable and the process is time consuming. In order to make more organized and repeatable phage films we use a programmable slide stainer machine (Carl Zeiss, Inc.) that can dip the slides into a each solution allowing time to dry and giving it the ability to agitate. Resultantly, we built phage films while varying soaking time, phage concentration, and the number of phage layers to determine the best conditions to form well-ordered phage films with the help of slide stainer.. Yang Xin. Paper presentation. PNAS 2009, 106(12):4629-4634. Introduction. Antimicrobial drug development is increasingly lagging behind the evolution of antibiotic resistance. as a result, there is a pressing need for new antibacterial therapies that can be readily designed. Megan . Mair. Mycobacteriophage. Giles. Genes Unique to Giles. Genes Conserved in Other Phages. 72%. 57 genes. 28%. 22 genes. A B. C D. F G. H I. J K. M N. P R. T Singleton. clusters. HHMI Logistics. . Name badges must be worn at all times. Restrooms. No roaming through the building. Questions? – Ask . us. Refreshments . (keep to the schedule). Technology. Live Streaming. The microphones. or phages are viruses that specifically infect bacteria. . Viral vectors are those in which gene of interest is incorporated in the genome of virus.. The phage particle attaches to the outer surface of bacterium and injects its DNA into the cell.. Dan Russell. Pauline: 7?!. Happy. Sad. 19. 2. Failure. Success. 19. 41. Star Trek. Star Wars. 9. 17. Batman. Superman. 8. 4. Mulan. Belle. 4. 4. Rapper. Scientist. 37. 43. 3. 5. 6. 10. 0. Mother. Father.