PPT-Transduction

Index Introduction Discovery Linkage data from transduction Specialized transduction Lytic and Lysogenic Cycle Introduction Transduction is the process by which DNA is transferred from one bacterium to another by a virus. (from Kimura, 1966). Electron micrograph of 3 OHCs. spiral ligament. modiolus. When the basilar membrane vibrates up and down in response to the sound wave, the hair cell cilia are displaced 1. st. in one direction then in the other; i.e., 1. Solutions Hexadimethrine Bromide (2 mg/ml stock solution) Product No. H9268 Puromycin (10 mg/ml stock solution) Product No. P9620 shRNA Many factors can affect transduction efficiency. Not all viral particles floating in culture medium can eventually transduce (or infect) the cells. Some additives such as polybrene can enhance the t Protein transduction technologyWadia and Dowdy 53       "    %& $   ()4,        Chapter 13, . Stryer. Short Course. Signal Transduction. Signal from exterior of cell must affect interior of cell. Through the membrane. Amplification of signal. Ability to turn off. A few pathways…. . Enzyme-linked . receptors. Nuclear receptors. Stochasticity. in . signalling. .. Enzyme-linked receptors. . signalling. through growth factors, hormones, cytokines. acting locally at very low concentrations (10. . Signal Transduction Pathway. EXTRACELLULAR. FLUID. Plasma membrane. CYTOPLASM. Receptor. Signaling. molecule: ligand. Relay molecules in a signal transduction pathway. Activation. Signal transduction . General features of signal transduction. Type of cell signalling. Type of Signal . transducer/Receptors. signal transduction. T. he . signal. . represents the information, . that is detected by specific . Believed to have evolved in prokaryotes and single-celled eukaryotes. Single-celled organisms. Bacteria use communication in quorum sensing. Short distance communication using local regulators. Secrete . Dr. Kevin Ahern. EGFR Signaling, Part 1. Epidermal Growth Factor Receptor (EGFR). EGFR Dimer. Autophosphorylated. Tyrosines in . Cytoplasmic Domain. Signaling Complex. Assembled on . Phosphotyrosines. More than 4 years experience in developing and analyzing mathematical models of complex bio-chemical reaction networks 10 Journal papers, 1 book chapter, 6 peer-reviewed conference papers, and 7 Z. Huang, Y. Chu, B. Cunha, and J. Hahn, Discovery. Linkage data from transduction. Specialized transduction. Lytic and Lysogenic Cycle. Introduction. Transduction is the process by which DNA. . is transferred from one bacterium to another by a virus.. Cellular Signaling. Autocrine Signals . Diffuse from one part of a cell to another part of the same cell. Synaptic signaling. Neurotransmitter is the signaling molecule. Occurs in the animal nervous system.