PPT-Chapter 12: Cloning by recombination

General background to sitespecific recombination The lambda recombination system more stealing from other organisms Potential advantages of using recombination for cloning Adaptation of the lambda recombination system for efficient cloning GATEWAY technology. (Part 1). Created by: Haley . Vrazel. Objectives. Define cloning.. Identify the history of cloning. . Analyze the different types of cloning.. What is Cloning?. Animal cloning is an assisted reproductive . A Brave New World. Over the centuries we have steadily expanded our understanding of, and control over, many aspects of the natural world but one natural process that largely eluded our mastery has been our own reproduction.. A Brave New World. Over the centuries we have steadily expanded our understanding of, and control over, many aspects of the natural world but one natural process that largely eluded our mastery has been our own reproduction.. In biotechnology, cloning refers to the different processes used for . duplicating . biological material . (ex. DNA fragments, cells or organisms). . . The 3 Types of Cloning Technologies:. 1. Recombinant DNA Technology/DNA Cloning. Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . Lecture . 26. th. Alexei Fedorov, 2014. Origin of Chess. http://www.expert-chess-strategies.com/who-invented-chess.html. http://www.ponderabout.com/archives/972/mind-boggling-exponential-growth.aspx. Alexei Fedorov. Recombination is an intrinsic property of nucleic acids and these molecules are susceptible to joining their ends. .. Evolution invented thousands of proteins that assist DNA recombination and became crucial for cell functioning. . Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . A number of different processes used to produce genetically identical copies of biological material. Researchers have cloned genes, cells, . tissues, and entire organisms. Do clones ever occur naturally?. :. Can you name at least four ways in which CSR and . V(D)J . recombination differ?. What are the substrates (what genes, what areas) for CSR and SHM? What are the key factors?. How does AID work? Where does it operate?. describe the production of natural clones in plants using the example of vegetative propagation in elm trees; . describe the production of artificial clones of plants from tissue culture;. discuss the advantages and disadvantages of plant cloning in agriculture (HSW6a, 6b, 7c); . Chapter 5: Investigating DNA. Major Topics. 5.1 DNA Isolation,  Sequencing, and Synthesis. 5.2 Bioinformatics. 5.3 Cloning and Recombinant Expression. 5.4 Microarrays. 5.5 In Situ Hybridization. Foundations of Biochemistry. Function . Part . 3. Cloning. Cloning means making an identical copy of something. In biology it can refer to. A lab method in which researchers copy fragments of DNA. A reproductive method in which researchers produce an exact genetic copy of an entire organism.. DEFINITION. HISTORY. STEPS . PROCEDURE. TYPES . EXAMPLES . ADVANTAGES AND DISADVANTAGES. WORD ORIGINATION. The term clone, invented by J. B. S. Haldane, is derived from the Ancient Greek word "twig", referring to the process whereby a new plant can be created from a twig..