PPT-DNA AMPLIFICATION . This lecture presents multiple methods for replicating a specific

Goal Reactions where the product grows exponentially with the number of cycles Main classes of methods 1 ThermalCycling PCR 2 Isothermal many methods Note If RNA is to be detected usually first transform to DNA. This can be generalized to any dimension brPage 9br Example of 2D gradient pic of the MATLAB demo Illustration of the gradient in 2D Example of 2D gradient pic of the MATLAB demo Gradient descent works in 2D brPage 10br 10 Generalization to multiple CS/. BioE. 598. Tandy Warnow. Alignment Error/Accuracy. SP-FN: percentage of homologies in the true alignment that are *not* recovered. SP-FP: percentage of homologies in the estimated alignment that are false. Hypothesis Tesing 2: Comparing Samples. Multiple Alignment. Stuart M. Brown. NYU School of Medicine. Learning Objectives. Understand the need for multiple alignment methods in biology. Optimal methods (dynamic programming) are not practical to align many sequences. Scott Walmsley, PhD. Research Instructor, Department Pharmaceutical Sciences. Skaggs School of Pharmacy. Outline . What is and why perform Multiple . S. equence . A. lignment (MSA)?. Pre-requisite knowledge. and Sequence Profiles. Multiple sequence alignment. Generalize our pairwise alignment of sequences to include . more than two. homologous proteins.. Looking at more than two sequences gives us . much more information. Tandy Warnow. The Department of Computer Science. The University of Texas at Austin. The “Tree of Life”. Avian Phylogenomics Project. G Zhang, . BGI. . Approx. 50 species, whole genomes. 8000+ genes, UCEs. Independent scientist. robert@drive5.com. www.drive5.com. Multiple . alingnment. and database search. Multiple alignment in 16S world. Curated. 16S multiple alignments:. RDP. SILVA. Greengenes. MSA methods for 16s:. The linear comparison of more than two sequences. Places residues in columns . per . position specific similarity scores . reflects . relationships . of the . sequences. the scores are based on . Tandy Warnow. Departments of Bioengineering and Computer Science. http://. tandy.cs.illinois.edu. O. r. a. ngut. a. n. G. orilla. Chimpanze. e. H. uma. n. F. r. om . the. . T. r. ee. . of. . the. L. Maya Schushan. November 2010. Outline- introduction to alignments. 1. . Introduction. 4. Pairwise Alignment:. Smith-Waterman. Needlman-Wunch. 5. Multiple Sequence Alignment:. ClustalW. MUSCLE. T-coffee. Tandy Warnow. Departments of Computer Science and Bioengineering. University of Illinois at Urbana-Champaign. Large-scale statistical phylogeny estimation. Ultra-large multiple-sequence alignment. Estimating species trees from incongruent gene trees. Places residues in columns . per . position specific similarity scores . reflects . relationships . of the . sequences. the scores are based on . indels. (gaps) and substitutions.. The alignment of residues implies that they have similar roles in the proteins or DNA sequences being aligned . Mark Ragan. Institute for Molecular Bioscience. and. . School of Information Technology & Electrical Engineering. The University of Queensland, Brisbane, Australia. IPAM Workshop on Multiple Sequence Alignment. the role of gene duplication followed by loss of function in speciation. For Friday, go through the slides from class 11. Aligning sequences is part of many analytical pipelines . Alignments can be local or global.