cromatest Kit Lab 4 Daheeya Alenazi Clinical significance Highest elevation of alkaline phosphatase ALP levels in serum Hepatobiliary disorders and Bone disease Paget disease osteitis deformans ID: 918791
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Slide1
ALKALINE PHOSPHATASE
Linear
cromatest
Kit
Lab 4
Daheeya Alenazi
Slide2Clinical significance
Highest elevation of alkaline phosphatase (ALP) levels in serum :
Hepatobiliary disorders and Bone disease.
Paget disease' (osteitis deformans).
bone cancer.
Normal ALP levels are age dependent and are elevated during periods of active bone growth.
Slide3An increase of up to 2-3 times normal is observed In the third trimester of pregnancy. Moderate elevations of ALP (not Involving the liver or bone) may be attributed to '
hodgkins
' disease, congestive heart failure, and abdominal bacterial Infections
Slide4Principle
p-
Nitrophenylphosphate
+ H
2
O
ALP &
Alkaline PH p-Nitrophenol + phosphateThe increase of absorbance for P-nitrophenol is directly proportional to the amount of ALP present in the sample
Slide5Procedure
Mix, incubate for 1 minute at RT.
Read initial absorbance at
405
nm of the sample, start the
the stopwatch and read absorbance for 3 min. WR preparation (1tablet 20mg+10ml of buffer)1mlSample
20 µl
Slide6Calculation
ΔA/min
X
2764 = …… U/L of ALP
Reference values at RT:
Adult : up to 180 U/L