Quantitative determination of alkaline phosphatase in Serum or heparinized plasma by a colorimetric kinetic method Daheeya AlEnazi Diagnostic Significance Highest elevation of alkaline ID: 776602
Download Presentation The PPT/PDF document " Lab 4 Alkaline Phosphatase(alp)" is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Slide1
Lab 4Alkaline Phosphatase(alp)
Quantitative determination of alkaline phosphatase in Serum or heparinized plasma by a colorimetric kinetic method.
Daheeya
AlEnazi
Slide2Diagnostic Significance
Highest elevation of alkaline
phosphatase (ALP
)
levels
in
serum
:
H
epatobiliary
disorders and
Bone
disease.
Paget disease
' (osteitis deformans).
bone cancer.
Normal
ALP levels are age dependent and are elevated during
periods of active bone growth.
Slide3An increase of up to 2-3 times normal is observed In the third trimester of pregnancy. Moderate elevations of ALP (not Involving the liver or bone) may
be
attributed to '
hodgkins
' disease, congestive heart failure, and abdominal bacterial Infections
Slide4PRINCIPLE
p-Nitrophenylphosphate + H2O Alkaline PH p-Nitrophenol + phosphateThe increase of absorbance for P-nitrophenol is directly proportional to the amount of ALP present in the sample
Slide5Procedure
Mix, incubate for 1 minute at 37 C
Read
initial
absorbance at405 nm
of the sample, start the
the
stopwatch
and read
absorbance
at 1 minute intervals thereafter for 3 minutes.
Calculate the difference between
absorbance
and the average absorbance differences per minute (
Δ
A/min)
Slide6Calculation
ΔA/min
X
2720 =
U/L of ALP
Reference values:
Men: 50 - 119 U/L
Women: 43 – 110 U/L