PPT-Eukaryotic Gene Finding

BMICS 776 wwwbiostatwiscedubmi776 Spring 2018 Anthony Gitter gitterbiostatwiscedu These slides excluding thirdparty material are licensed under CC BYNC 40 by Mark Craven Colin Dewey and Anthony Gitter. TSW – Recognize that the presence of a nucleus determines whether a cell is prokaryotic or eukaryotic. 6.12B. Cells. There are two . basic types . of cells. Prokaryotes. or prokaryotic cells . Eukaryotes. Reece . Andonov. Lizzie . Codina. Period 3. Nucleus . vs. Prison Warden. Found in both types of eukaryotic cells. The nucleus is like the prison warden because the warden controls all aspects of the prison and the nucleus controls all functions of the cell, also they are both the most important.. Kingdom Protista. A. Sometimes not considered a true kingdom because the organisms vary tremendously from one to another. The only universal characteristic among the group is that they are all eukaryotic.. Discuss distinguishing characteristics of the domains and kingdoms of living organisms.. Classify organisms based on the distinguishing characteristics of the domains and/or kingdoms of living organisms.. 2. Terminology . Genome. – entire genetic material of an individual. Transcriptome. – set of transcribed sequences. Proteome. – set of proteins encoded by the genome. . Gene. * Basic . physical and functional units of heredity.. Two Basic Types. Remember….cells are the basic unit of life for ALL living things.. There are two basic types of cells:. Prokaryotic cells . – found in bacteria. Eukaryotic cells . – found in . Unit. Context of Unit in Course . Unit is for a second year, intro cell biology or intro microbiology course, scalable to first year intro biology. This . unit comes in . the middle . of . the course. Eukaryotic Cell. 3. Eu. = True. Karyon. = Nucleus. B- The Eukaryotic Cell. Animal Cell. Plant Cell. Compare Animal and Plant cell. What are the functions of cell organelles . ?. Copyright © 2002 Pearson Education, Inc., publishing as Benjamin Cummings. Draw 8 boxes on your paper. Gene regulation accounts for some of the phenotypic differences between organisms with similar genes.. 2005-2006. Gene regulation in bacteria. Control of gene expression enables individual bacteria to adjust their metabolism to environmental change. Gene Expression. Gene Expression. Transcription. Splicing. Polyadenylation. mRNA Stability. Translation. Protein Stability. Controls on Protein Levels. Transcription Control - Prokaryotic Promoter. Transcription Control - Prokaryotic Promoter. Eukaryotic translation. Eukaryotic mRNAs are characterized by two post-transcriptional modifications: the 5-terminal 7methyl-GTP cap and the 3-terminal poly(A) tail .. The 7methyl-GTP cap is essential for mRNA binding by eukaryotic . Lecture 3. Gene Finding and Sequence Annotation. Objectives of this lecture. Introduce you to basic concepts and approaches of gene finding. Show you differences between gene prediction for prokaryotic and eukaryotic genomes. A single guiding RNA (sgRNA; . a fusion of . tracrRNA. and crRNA. ) . binds CAS9 protein at one end, the other end guides CAS9 to target DNA sequence based on complementarity. . Gene for the CAS9 protein, encodes endonuclease, cuts both strands of the target sequence. Biology / Bioinformatics . . Edward . Marcotte. , . Univ. of Texas at Austin. Lots of genes in every genome. Nature Reviews Genetics . 13:329-342 (2012). Do humans really have the biggest genomes?.