Gene xpert ve 75 64 Live bacilli 37 Pulm amp 48 EPTB Smear amp Culture ve Gene xpert ve 37 32 Dead bacilli Smear amp Culture ve ID: 776521
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Slide1
Smear + / -Culture +veGene xpert + ve75 (64%)(Live bacilli)(37 Pulm & 48 EPTB)Smear & Culture - veGene xpert +ve37 (32%)(Dead bacilli)Smear & Culture + veGene xpert -ve5 (4%)(Atypical Mycobacteria)
GeneXpert MTB/RIF
assay(n=117)
Slide2GENE XPERT PERFORMANCE SPUTUM (n = 54)
Gold StandardGene xpert+veGene xpert - veCulture+ve15 (T+ve)0 (F-ve) Aty MbCulture -ve5 (F +ve) Dead34 (T –ve)
Slide3GENE XPERT PERFORMANCE BRONCHIAL WASH (n = 52)
Gold StandardGene xpert+veGene xpert - veCulture+ve12 (T+ve)0 (F-ve) Aty MbCulture -ve3 (F +ve) Dead37 (T –ve)
Slide4GENE XPERT PERFORMANCE Extra-Pulmonary Samples (n=153)
Specimen
No.
C+
ve
GeX
+
ve
Sensitivity (%)
Specificity(%)
PPV(%)
NPV (%)
CSF
78
4
100
89.19
33.3
100
Asp. Pus
26
18
100
52.63
43.75
100
Tissue
(bon)
23
11
100
78.95
50
100
Lymph node
26
15
100
63.64
33.3
100
Slide5Extra-Pulmonary Samples –Culture & GeneXpert Negative (n=61)
SPECIMEN
NO.
Pleural
fluid
26
Ascitic fluid
22
Synovial fluid
6
Aspirated fluid
5
Pericardial
fluid
1
Peritoneal fluid
1
Slide6GENE XPERT PERFORMANCE-Sensitivity
Analytical sensitivity
5
genome copies of purified DNA
131 CFU/ml
of
M.tb
Sensitivity =
100%
Negative Predictive value =
100%
Test accuracy =
100%
(detected all smear +
ve
/ Culture +
ve
)
Slide7GENE XPERT PERFORMANCE -Specificity
SpecimenSpecificity (%)PPV (%)Sputum8775Bronchial wash9380CSF9033 Asp. Pus5344Tissue (bone)7950Lymph node6433
32%
Dead bacilli - Already treated cases
??