PPT-Lab – 5 Bacterial Smear Preparation & Staining

Bacterial Smear Preparation It is a preparation that includes a small amount of biological sample or culture spread in a very thin film on the surface of the slide Smear preparation is a step before staining. A well Made and well Stained Smear can provide: . Estimates of cell count. Proportions of the different types of WBC. Morphology. Peripheral Blood Smear. Objective. 1. Specimen Collection. 2. Peripheral Smear Preparation. HAEMATOXYLIN & EOSIN. . Supervised by : . Dr.Sherifa. . Prepared by: Reem Aldossari. Lama . Alabdi. H&E staining:. The best known and most widely used staining procedure, . hematoxylin. -eosin staining (H&E), uses . Lab #4 . Medgar Evers College. Bio 261 Microbiology. Prof. Santos. Aim #1 exercise 14. Capsular staining. Capsule- extracellular layer composed of polysaccharides found in some bacteria that plays a role in immunity and adhesion.. Pure Culture Techniques. **. Turn on Incinerators and hot plates (low heat) . **. Microbial Culture. M. ethod of multiplying microbial organisms by letting them reproduce in a predetermined culture media under controlled laboratory conditions. By . Dr.N.Sundari. What is the role of pap smear in cervical cancer screening presently ??. Gold standard test for cervical cancer screening. Obsolete & historic importance ??. Eliminating cytology greatly simplifies screening – Cytology adds very little to the initial . A.L. Noor Ameer . The reasons of staining. 1- To study their shapes . 2- . To differentiate the species of bacteria by using differential . stain.. 3- . To study the internal components of the bacterial . . Bacterial staining . Lab. 5 . A.L. Noor Ameer. Purpose of culturing is: . 1- Isolation of microorganisms from a . sample.. 2- . For counting the microorganism in the . sample.. 3- . Obtain pure . & . Antibiotic susceptibility testing. : An introduction. Dr. Mohit Bhatia. Assistant Professor. Department of Microbiology. AIIMS, Rishikesh. Staining techniques . Smear preparation. Smear = 2 × 3 cm in size. . DR. SANJIV KUMAR. ASSTT. PROFESSOR,. DEPTT. OF PATHOLOGY, BVC, PATNA. CONTENTS. INTRODUCTION. APPLICATIONS. TECHNIQUES. SMEAR FORMATION. FIXATION. STAINING METHODS. INTERPRETATIONS. INTRODUCTION. Is . tissues, cells, cell components, cell contents, or microorganisms.. *The stain may be . natural or synthetic. . . *The object stained is called the . substrate. . . *Staining facilitates the observation of a substrate by introducing . Sethi S, Alcid D, Kesarwala H, Tolan RW. Probable Congenital Babesiosis in Infant, New Jersey, USA. Emerg Infect Dis. 2009;15(5):788-791. https://doi.org/10.3201/eid1505.070808. Cervical cancer. Commonest form of cervical cancer is Squamous Cell Carcinoma. Strong correlation between HPV and smoking. Week co-factors: oncogenes, lower immune response and oral contraceptive pill (COC). Fixatives used in histopathology. 2. Staining. Differential staining. Gram’s staining. Vital staining. Trypan. blue- dead cells take up the stain . i.e. stain positively and live cells . Exclude the stain or stain negatively. 5/29/2023. 1. Practical Bacteriology. lab. . 2. By:. Asst. Prof. Shiama'a Al-Salihy. Learning objectives:. 5/29/2023. 2. After this lab, you must be able to:. Identify laboratory diagnosis steps.. Understand staining techniques.