PPT-Lab – 5 Bacterial Smear Preparation & Staining

Bacterial Smear Preparation It is a preparation that includes a small amount of biological sample or culture spread in a very thin film on the surface of the slide Smear preparation is a step before staining. A well Made and well Stained Smear can provide: . Estimates of cell count. Proportions of the different types of WBC. Morphology. Peripheral Blood Smear. Objective. 1. Specimen Collection. 2. Peripheral Smear Preparation. A stain is a substance that adheres to a cell, giving the cell color. .. The presence of color gives the cells significant contrast so are much more visible. . Different stains have different affinities for different organisms, or different parts of . Practical Hematology Lab. Making Blood Smear. - LAB 1 -. I- Preparation Of Blood Smear. There are three types of blood smears: . The cover glass smear.. The wedge smear .. The spun smear.. The are two additional types of blood smear used for specific purposes. Staining. Coloring organism with a dye. Microorganisms must be . FIXED. to microscopic slide first. Kills microbe. Attaches organism to the slide. Preserves various parts with minimal distortion. Steps to fix:. A.L. Noor Ameer . The reasons of staining. 1- To study their shapes . 2- . To differentiate the species of bacteria by using differential . stain.. 3- . To study the internal components of the bacterial . I- Preparation Of Blood Smear. There are three types of blood smears: . The cover glass smear.. The wedge smear .. The spun smear.. The are two additional types of blood smear used for specific purposes. Introduction . Direct . fecal . smears. Direct . fecal smears can be used as a quick screening test to check for any intestinal . parasite.. Advantages . &Disadvantages . Advantages:-. U. seful . Bacteria: are unicellular free living organisms without . chlorophil. having . b. oth DNA and RNA . Bacterial . morphology. Can . be grouped into three types . 1- Spherical . (. cocci. ). 2- Rod – . By. Assistant lecturer. Zainab farooq . shafeeq. Bacterial Morphology. Bacteria: are unicellular free living organisms without . chlorophil. having doth . DNAand. RNA . Bacterial . morphology. Can . tissues, cells, cell components, cell contents, or microorganisms.. *The stain may be . natural or synthetic. . . *The object stained is called the . substrate. . . *Staining facilitates the observation of a substrate by introducing . العملي. Lab -1. -2-. . Bacterial Taxonomy. Bacteria are classified and identified to distinguish among strains and to group them by criteria of interest to microbiologists and other scientists.. . Essam. Bacterial Morphology. Bacteria: are unicellular free living organisms without . chlorophil. having . b. oth DNA and RNA . Bacterial . morphology. Can . be grouped into three types . 1- Spherical . This. procedure is called . so,when. certain bacteria or some of their . structures could not be stained or seen or being differential with other bacteria cells when using the ordinary . staining,such. Fixatives used in histopathology. 2. Staining. Differential staining. Gram’s staining. Vital staining. Trypan. blue- dead cells take up the stain . i.e. stain positively and live cells . Exclude the stain or stain negatively.